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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Eichmann, TO; Grumet, L; Taschler, U; Hartler, J; Heier, C; Woblistin, A; Pajed, L; Kollroser, M; Rechberger, G; Thallinger, GG; Zechner, R; Haemmerle, G; Zimmermann, R; Lass, A.
ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6.
J Lipid Res. 2015; 56(10):1972-84 Doi: 10.1194/jlr.M062372 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Führende Autor*innen der Med Uni Graz
Eichmann Thomas
Co-Autor*innen der Med Uni Graz
Hartler Jürgen
Kollroser Manfred
Taschler Ulrike

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Lipid droplets (LDs) of hepatic stellate cells (HSCs) contain large amounts of vitamin A [in the form of retinyl esters (REs)] as well as other neutral lipids such as TGs. During times of insufficient vitamin A availability, RE stores are mobilized to ensure a constant supply to the body. To date, little is known about the enzymes responsible for the hydrolysis of neutral lipid esters, in particular of REs, in HSCs. In this study, we aimed to identify LD-associated neutral lipid hydrolases by a proteomic approach using the rat stellate cell line HSC-T6. First, we loaded cells with retinol and FAs to promote lipid synthesis and deposition within LDs. Then, LDs were isolated and lipid composition and the LD proteome were analyzed. Among other proteins, we found perilipin 2, adipose TG lipase (ATGL), and comparative gene identification-58 (CGI-58), known and established LD proteins. Bioinformatic search of the LD proteome for α/β-hydrolase fold-containing proteins revealed no yet uncharacterized neutral lipid hydrolases. In in vitro activity assays, we show that rat (r)ATGL, coactivated by rat (r)CGI-58, efficiently hydrolyzes TGs and REs. These findings suggest that rATGL and rCGI-58 are LD-resident proteins in HSCs and participate in the mobilization of both REs and TGs.
Find related publications in this database (using NLM MeSH Indexing)
Acyltransferases - metabolism
Adipocytes - metabolism
Animals - administration & dosage
Cell Line - administration & dosage
Hepatic Stellate Cells - metabolism
Lipase - metabolism
Lipid Droplets - metabolism
Lipolysis - administration & dosage
Lipoproteins - metabolism
Membrane Proteins - metabolism
Perilipin-2 - administration & dosage
Proteome - metabolism
Rats - administration & dosage
Retinol-Binding Proteins - metabolism
Retinyl Esters - administration & dosage
Triglycerides - metabolism
Vitamin A - pharmacology

Find related publications in this database (Keywords)
neutral lipid hydrolase
adipose triglyceride lipase
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