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Orzińska, A; Guz, K; Polin, H; Pelc-Kłopotowska, M; Bednarz, J; Gieleżyńska, A; Sliwa, B; Kowalewska, M; Pawłowska, E; Włodarczyk, B; Malaga Alicja Żmudzin, M; Krzemienowska, M; Srivastava, K; Michalewska, B; Gabriel, C; Flegel, WA; Brojer, E.
RHD variants in Polish blood donors routinely typed as D-.
Transfusion. 2013; 53(11 Suppl 2): 2945-2953. Doi: 10.1111/trf.12230 [OPEN ACCESS]
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GABRIEL Christian

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Blood donors exhibiting a weak D or DEL phenotypical expression may be mistyped D- by standard serology hence permitting incompatible transfusion to D- recipients. Molecular methods may overcome these technical limits. Our aim was to estimate the frequency of RHD alleles among the apparently D- Polish donor population and to characterize its molecular background. Plasma pools collected from 31,200 consecutive Polish donors typed as D- were tested by real-time polymerase chain reaction (PCR) for the presence of RHD-specific markers located in Intron 4 and Exons 7 and 10. RHD+ individuals were characterized by PCR or cDNA sequencing and serology. Plasma cross-pool strategy revealed 63 RHD+ donors harboring RHD*01N.03 (n = 17), RHD*15 (n = 12), RHD*11 (n = 7), RHD*DEL8 (n = 3), RHD*01W.2 (n = 3), RHD-CE(10) (n = 3), RHD*01W.3, RHD*01W.9, RHD*01N.05, RHD*01N.07, RHD*01N.23, and RHD(IVS1-29G>C) and two novel alleles, RHD*(767C>G) (n = 3) and RHD*(1029C>A). Among 47 cases available for serology, 27 were shown to express the D antigen 1) Plasma cross-pool strategy is a reliable and cost-effective tool for RHD screening. 2) Only 0.2% of D- Polish donors carry some fragments of the RHD gene; all of them were C or E+. 3) Almost 60% of the detected RHD alleles may be potentially immunogenic when transfused to a D- recipient. © 2013 American Association of Blood Banks.
Find related publications in this database (using NLM MeSH Indexing)
Alleles -
Blood Donors - statistics & numerical data
Blood Grouping and Crossmatching - economics Blood Grouping and Crossmatching - statistics & numerical data
Blood Transfusion - statistics & numerical data
Cost-Benefit Analysis -
Diagnostic Tests, Routine - economics Diagnostic Tests, Routine - statistics & numerical data
Genetic Variation -
Humans -
Molecular Sequence Data -
Phenotype -
Phenotype - epidemiology
Reproducibility of Results -
Rh-Hr Blood-Group System - genetics Rh-Hr Blood-Group System - immunology
Serologic Tests - economics
Validation Studies as Topic -

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