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SHR Neuro Krebs Kardio Lipid

Hildner, F; Peterbauer, A; Wolbank, S; Nürnberger, S; Marlovits, S; Redl, H; van Griensven, M; Gabriel, C.
FGF-2 abolishes the chondrogenic effect of combined BMP-6 and TGF-beta in human adipose derived stem cells.
J Biomed Mater Res A. 2010; 94(3): 978-987.
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Autor/innen der Med Uni Graz:
Gabriel Christian
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Abstract:
In this study, we investigated the influence of transforming growth factor beta 3 (TGF-beta3), bone morphogenetic protein 6 (BMP-6) and basic fibroblast growth factor (FGF-2) on chondrogenesis in adipose derived stem cells (ASC). Cells were isolated from liposuction material, expanded and subjected to chondrogenic differentiation. Micromass pellets were cultured in chondrogenic medium containing 10 ng/mL TGF-beta3 which was additionally supplemented with 10 ng/mL BMP-6, 10 ng/mL FGF-2 or a combination of both. We quantitatively evaluated the cartilage specific gene expression after 14 days of culture. The end point measurements on day 35 included glycosaminoglycan (GAG) quantification, histological staining for chondrogenic markers, and transmission electron microscopy (TEM). In comparison to cultures induced with TGF-beta3/FGF-2, the presence of TGF-beta3/BMP-6 demonstrated strong induction of collagen type II, collagen type IX and aggrecan mRNA expression. This was corroborated by quantification and histological staining for GAGs and immunohistological staining for collagen II. However, when a combination of BMP-6 and FGF-2 in addition to TGF-beta3 was added, FGF-2 counteracted BMP-6, as indicated by reduced marker gene expression and weak to absent staining for GAGs. In conclusion, this study demonstrates that BMP-6 combined with TGF-beta3 is a potent inducer of chondrogenesis in human ASC. In contrast, FGF-2 does not contribute to differentiation, but rather suppresses the chondrogenic potential of BMP-6. (c) 2010 Wiley Periodicals, Inc.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Animals -
Biological Markers - metabolism
Bone Morphogenetic Protein 6 - pharmacology
Cell Differentiation - drug effects
Cells, Cultured -
Chondrogenesis - drug effects
Culture Media - chemistry
Female -
Fibroblast Growth Factor 2 - pharmacology
Glycosaminoglycans - metabolism
Humans -
Middle Aged -
RNA - metabolism
Stem Cells - cytology Stem Cells - drug effects Stem Cells - physiology
Transforming Growth Factor beta3 - pharmacology

Find related publications in this database (Keywords)
adipose derived stem cells
cartilage
growth factors
FGF-2
BMP-6
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