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SHR Neuro Krebs Kardio Lipid

Sahu-Osen, A; Montero-Moran, G; Schittmayer, M; Fritz, K; Dinh, A; Chang, YF; McMahon, D; Boeszoermenyi, A; Cornaciu, I; Russell, D; Oberer, M; Carman, GM; Birner-Gruenberger, R; Brasaemle, DL.
CGI-58/ABHD5 is phosphorylated on Ser239 by protein kinase A: control of subcellular localization.
J Lipid Res. 2015; 56(1):109-121 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Birner-Grünberger Ruth
Fritz Katarina
Sahu-Osen Anita
Schittmayer-Schantl Matthias

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Number of Figures: 6
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CGI-58/ABHD5 coactivates adipose triglyceride lipase (ATGL). In adipocytes, CGI-58 binds to perilipin 1A on lipid droplets under basal conditions, preventing interaction with ATGL. Upon activation of protein kinase A (PKA), perilipin 1A is phosphorylated and CGI-58 rapidly disperses into the cytoplasm, enabling lipase coactivation. Because the amino acid sequence of murine CGI-58 has a predicted PKA consensus sequence of RKYS(239)S(240), we hypothesized that phosphorylation of CGI-58 is involved in this process. We show that Ser239 of murine CGI-58 is a substrate for PKA using phosphoamino acid analysis, MS, and immuno-blotting approaches to study phosphorylation of recombinant CGI-58 and endogenous CGI-58 of adipose tissue. Phosphorylation of CGI-58 neither increased nor impaired coactivation of ATGL in vitro. Moreover, Ser239 was not required for CGI-58 function to increase triacylglycerol turnover in human neutral lipid storage disorder fibroblasts that lack endogenous CGI-58. Both CGI-58 and S239A/S240A-mutated CGI-58 localized to perilipin 1A-coated lipid droplets in cells. When PKA was activated, WT CGI-58 dispersed into the cytoplasm, whereas substantial S239A/S240A-mutated CGI-58 remained on lipid droplets. Perilipin phosphorylation also contributed to CGI-58 dispersion. PKA-mediated phosphorylation of CGI-58 is required for dispersion of CGI-58 from perilipin 1A-coated lipid droplets, thereby increasing CGI-58 availability for ATGL coactivation. Copyright © 2015 by the American Society for Biochemistry and Molecular Biology, Inc.
Find related publications in this database (using NLM MeSH Indexing)
1-Acylglycerol-3-Phosphate O-Acyltransferase - chemistry
1-Acylglycerol-3-Phosphate O-Acyltransferase - metabolism
Adipocytes - cytology
Adipocytes - drug effects
Adipocytes - metabolism
Amino Acid Sequence -
Animals -
COS Cells -
Carrier Proteins - metabolism
Cercopithecus aethiops -
Colforsin - pharmacology
Cyclic AMP-Dependent Protein Kinases - metabolism
Gene Expression Regulation - drug effects
Humans -
Intracellular Space - drug effects
Intracellular Space - metabolism
Lipase - metabolism
Male -
Mice -
Molecular Sequence Data -
Perilipin-1 -
Phosphoproteins - metabolism
Phosphorylation - drug effects
Protein Binding - drug effects
Protein Transport - drug effects
Recombinant Proteins - chemistry
Recombinant Proteins - metabolism
Serine - metabolism

Find related publications in this database (Keywords)
adipose tissue
adipose triglyceride lipase
Chanarin Dorfman syndrome
lipid droplets
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