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Wultsch, G; Nersesyan, A; Kundi, M; Jakse, R; Beham, A; Wagner, KH; Knasmueller, S.
The sensitivity of biomarkers for genotoxicity and acute cytotoxicity in nasal and buccal cells of welders.
Int J Hyg Environ Health. 2014; 217(4-5):492-498
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Autor/innen der Med Uni Graz:
Beham Alfred
Wultsch Georg
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Abstract:
Welders are inhalatively exposed to fumes which contain genotoxic carcinogens and it was found in epidemiological studies that they have increased cancer rates which may be causally related to DNA damage. In order to assess their health risks and to find out which chemicals cause the adverse effects, bioassays can be performed which enable the detection of genetic damage. The aim of the present study was a comparative investigation with exfoliated buccal and nasal cells in regard to induction of chromosomal alterations and acute cytotoxicity in welders and unexposed controls (n=22 per group). To elucidate the factors which account for genotoxic and cytotoxic effects, additional biochemical parameters were monitored reflecting the redox status as well as concentrations of different metals and 1-hydroxypyrene (1-OHP) in body fluids. We found in the nasal cells significant induction of alterations which are indicative for DNA damage, i.e. of micronuclei (MNi) and nuclear buds, while elevated rates of nuclear anomalies reflecting cytotoxic effects (condensed chromatin, karyorrhexis, karyolylsis) were detected in cells from both organs. The levels of certain metals (Cr, Cu, Mn, Mo, Ni), but not markers of oxidative damage were significantly higher in the body fluids of the welders. Multivariate Poisson regression analyses indicate that exposure to Mo (15% MNi increase by one standard deviation increase of Mo in serum), Ni (9% increase) and Mn (14% increase) are positively associated with the induction of MNi in nasal cells while Ni was associated with cytotoxic effects in both types of cells (12 and 16% increase). Taken together, our findings indicate that epithelial cells from the respiratory tract are suitable for the detection of DNA-damaging and cytotoxic effects in welders and can be used to assess health risks associated with genomic instability. Copyright © 2013 Elsevier GmbH. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Biological Markers - metabolism
Cells, Cultured -
DNA Damage -
Genomic Instability -
Humans -
Metals, Heavy - blood Metals, Heavy - toxicity
Mouth Mucosa - drug effects Mouth Mucosa - metabolism
Multivariate Analysis -
Mutagenicity Tests -
Nasal Mucosa - drug effects Nasal Mucosa - metabolism
Occupational Exposure -
Poisson Distribution -
Welding -

Find related publications in this database (Keywords)
Welders
Buccal cells
Nasal cells
Cytome assay
Heavy metals
Redox status
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