Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Cacciatore, S; Hu, X; Viertler, C; Kap, M; Bernhardt, GA; Mischinger, HJ; Riegman, P; Zatloukal, K; Luchinat, C; Turano, P.
Effects of intra- and post-operative ischemia on the metabolic profile of clinical liver tissue specimens monitored by NMR.
J Proteome Res. 2013; 12(12):5723-5729
Doi: 10.1021/pr400702d
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Bernhardt Gerwin
- Mischinger Hans-Jörg
- Viertler Christian
- Zatloukal Kurt
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- Abstract:
- Metabolomic profiles of tissues could greatly contribute to advancements in personalized medicine but are influenced by differences in adopted preanalytical procedures; nonhomogeneous pre- and post-excision ischemia times are potential sources of variability. In this study, we monitored the impact of ischemia on the metabolic profiles, acquired with high-resolution magic-angle-spinning (1)H NMR, of 162 human liver samples collected during and up to 6 h after routine surgery. The profiles changed significantly as a function of intraoperative warm ischemia (WI) and postresection cold ischemia (CI) time, with significant variations in the concentration of the same 16 metabolites. Therefore, a tight control of the preanalytical phase is essential for reliable metabolomic analyses of liver diseases. The NMR profiles provide a reliable "fingerprint" of ischemia and have predictive value: the best-performing predictive models are found to discriminate extreme time points of CI (0' vs 360 ') in the training set with cross-validation accuracy of ~90%; samples in the validation cohort can discriminate short (≤60') from long (≥180') CI with an accuracy of ~80%. For WI, the corresponding figures are 95.6 and 92%, respectively. Therefore, ischemia NMR profiles might become a tool for tissue quality control in biobanks.
- Find related publications in this database (using NLM MeSH Indexing)
- Biological Markers - metabolism
- Carcinoma - metabolism
- Cold Ischemia -
- Colonic Neoplasms - metabolism
- Humans -
- Liver - metabolism
- Liver Neoplasms - metabolism
- Magnetic Resonance Spectroscopy -
- Metabolome -
- Models, Statistical -
- Time Factors -
- Warm Ischemia -
- Find related publications in this database (Keywords)
- tissue metabolomics
- ischemia
- NMR
- HR-MAS
- liver
- preanalytics