Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid Stoffw Microb

Lapolla, A; Porcu, S; Roverso, M; Desoye, G; Cosma, C; Nardelli, GB; Bogana, G; Carrozzini, M; Traldi, P.
A preliminary investigation on placenta protein profile reveals only modest changes in well controlled gestational diabetes mellitus.
Eur J Mass Spectrom (Chichester). 2013; 19(3):211-223 Doi: 10.1255/ejms.1225
Web of Science PubMed FullText FullText_MUG


Co-Autor*innen der Med Uni Graz
Desoye Gernot

Dimensions Citations:

Plum Analytics:

Scite (citation analytics):

Gestational diabetes mellitus (GDM) is associated with a wide range of tissue-specific changes depending on the quality of glycemic control of the mothers. Here we tested the hypothesis that GDM is associated with alterations in the human term placenta proteome. For this aim, two different approacheswere employed. The placenta homogenates from 20 healthy subjects and those from 20 GDM pregnant women were pooled. The two samples thus obtained were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and the proteins detected were tentatively identified by comparison of their molecular weight with the Human Protein Reference Database, restricting the search to the species expressed in the placenta tissue. However this approach led to misleading results: in fact, an in deep analysis of the spectra and tandem mass spectrometry (MS/MS) measurements of the digestion products from the protein detected, unequivocally proved that the species observed are maternal and fetal globins. Consequently, the two pools were analyzed by 1D sodium dodecyl sulphate polyacrylamide gel electrophoresis; the different bands obtained were digested by trypsin and the digestion products were analyzed by MALDI-MS; the protein identification was carried out by comparison of the peptide mass fingerprint with databases. Only modest quantitative differences were observed between the placenta protein profiles of healthy and GDM subjects, indicating that GDM, if well controlled, induces only minor changes in the placental proteome. One example of differently expressed proteins in the placenta homogenate pool from GDM and the controls was the SRRM1 protein, a member of the serine-arginine protein kinase family; for GDM samples, the MALDI spectrum of its digestion products showed the presence of molecular species attributable to glycation and glyco-oxidation processes.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Diabetes, Gestational - metabolism
Electrophoresis, Gel, Two-Dimensional - methods
Female -
Glycosylation -
Humans -
Oxidation-Reduction -
Peptide Mapping - methods
Placenta - chemistry
Placenta - metabolism
Pregnancy -
Pregnancy Proteins - analysis
Pregnancy Proteins - metabolism
Proteome - analysis
Proteome - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

Find related publications in this database (Keywords)
placenta proteome
gestational diabetes
MALDI (matrix-assisted laser desorption ionization) mass spectrometry
© Med Uni Graz Impressum