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SHR Neuro Krebs Kardio Lipid

Kirsch, AH; Riegelbauer, V; Tagwerker, A; Rudnicki, M; Rosenkranz, AR; Eller, K.
The mTOR-inhibitor rapamycin mediates proteinuria in nephrotoxic serum nephritis by activating the innate immune response.
Am J Physiol Renal Physiol. 2012; 303(4):F569-F575 [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Eller Kathrin
Kirsch Alexander
Rosenkranz Alexander
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Abstract:
Rapamycin (Rapa) is an immunosuppressant used to prevent rejection in recipients of renal transplants. Its clinical use is limited by de novo onset or exacerbation of preexisting proteinuria. In the present study, Rapa administration was started 14 days after induction of murine nephrotoxic serum nephritis (NTS) to study glomerular effects of this mammalian target of rapamycin (mTOR) inhibitor. Glomeruli were laser-microdissected, and real-time PCR was performed to assess effects on glomerular cells and the expression of inflammatory cytokines. Immunohistochemical stainings were performed to confirm mRNA data on the protein level. Compared with nephritic control animals, Rapa-treated mice developed significantly increased albuminuria. This was accompanied by a more prominent glomerular infiltration by CD4(+) T cells and macrophages. Glomerular mRNA expression profiling revealed increased levels of the proinflammatory cytokines interleukin-6 and tumor necrosis factor-α, and the chemokines monocyte chemoattractant protein-1 and macrophage inflammatory protein-1β and their cognate macrophage-associated receptors CCR2 and CCR5 in the Rapa-treated animals. Furthermore, there were elevated glomerular transcription levels of the regulatory T cell phenotype transcription factor Foxp3. No differences in the glomerular expression of the podocyte marker nephrin or the endothelial cell marker CD31 were observed on the mRNA or protein level. In conclusion, our data indicate that Rapa-induced proteinuria in NTS is a result of the activation of the innate immune system rather than a direct toxicity to podocytes or glomerular endothelial cells.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Antigens, CD31 - genetics
Gene Expression Regulation - drug effects
Immunity, Innate - drug effects
Inflammation Mediators - metabolism
Male -
Membrane Proteins - genetics
Mice -
Mice, Inbred C57BL -
Nephritis - chemically induced
Proteinuria - metabolism
RNA, Messenger -
Sirolimus - pharmacology
TOR Serine-Threonine Kinases - antagonists & inhibitors
Vascular Endothelial Growth Factor A - genetics

Find related publications in this database (Keywords)
mammalian target of rapamycin inhibition
rapamycin
glomerulonephritis
proteinuria
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