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SHR Neuro Krebs Kardio Lipid

Dichlberger, A; Schlager, S; Lappalainen, J; Käkelä, R; Hattula, K; Butcher, SJ; Schneider, WJ; Kovanen, PT.
Lipid body formation during maturation of human mast cells.
J Lipid Res. 2011; 52(12):2198-2208 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Schlager Stefanie

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Number of Figures: 9
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Lipid droplets, also called lipid bodies (LB) in inflammatory cells, are important cytoplasmic organelles. However, little is known about the molecular characteristics and functions of LBs in human mast cells (MC). Here, we have analyzed the genesis and components of LBs during differentiation of human peripheral blood-derived CD34(+) progenitors into connective tissue-type MCs. In our serum-free culture system, the maturing MCs, derived from 18 different donors, invariably developed triacylglycerol (TG)-rich LBs. Not known heretofore, the MCs transcribe the genes for perilipins (PLIN)1-4, but not PLIN5, and PLIN2 and PLIN3 display different degrees of LB association. Upon MC activation and ensuing degranulation, the LBs were not cosecreted with the cytoplasmic secretory granules. Exogenous arachidonic acid (AA) enhanced LB genesis in Triacsin C-sensitive fashion, and it was found to be preferentially incorporated into the TGs of LBs. The large TG-associated pool of AA in LBs likely is a major precursor for eicosanoid production by MCs. In summary, we demonstrate that cultured human MCs derived from CD34(+) progenitors in peripheral blood provide a new tool to study regulatory mechanisms involving LB functions, with particular emphasis on AA metabolism, eicosanoid biosynthesis, and subsequent release of proinflammatory lipid mediators from these cells.
Find related publications in this database (using NLM MeSH Indexing)
Antigens, CD34 - metabolism
Arachidonic Acids - pharmacology
Cell Degranulation - drug effects
Cell Differentiation - drug effects
Cytoplasm - drug effects Cytoplasm - metabolism
Gene Expression Regulation - drug effects
Humans -
Lipid Metabolism - drug effects
Lipids - chemistry
Mast Cells - cytology Mast Cells - drug effects Mast Cells - metabolism
Membrane Proteins - genetics
Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism
Triglycerides - metabolism
Vesicular Transport Proteins - genetics

Find related publications in this database (Keywords)
arachidonic acid
fatty acid
secretory granules
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