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SHR Neuro Krebs Kardio Lipid

Haybaeck, J; Stumptner, C; Thueringer, A; Kolbe, T; Magin, TM; Hesse, M; Fickert, P; Tsybrovskyy, O; Müller, H; Trauner, M; Zatloukal, K; Denk, H.
Genetic background effects of keratin 8 and 18 in a DDC-induced hepatotoxicity and Mallory-Denk body formation mouse model.
Lab Invest. 2012; 92(6):857-867 [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Denk Helmut
Fickert Peter
Haybäck Johannes
Müller Heimo
Stumptner Cornelia
Trauner Michael
Tsybrovskyy Oleksiy
Zatloukal Kurt
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Abstract:
Keratin 8 (K8) and keratin 18 (K18) form the major hepatocyte cytoskeleton. We investigated the impact of genetic loss of either K8 or K18 on liver homeostasis under toxic stress with the hypothesis that K8 and K18 exert different functions. krt8⁻/⁻ and krt18⁻/⁻ mice crossed into the same 129-ola genetic background were treated by acute and chronic administration of 3,5-diethoxy-carbonyl-1,4-dihydrocollidine (DDC). In acutely DDC-intoxicated mice, macrovesicular steatosis was more pronounced in krt8⁻/⁻ and krt18⁻/⁻ compared with wild-type (wt) animals. Mallory-Denk bodies (MDBs) appeared in krt18⁻/⁻ mice already at an early stage of intoxication in contrast to krt8⁻/⁻ mice that did not display MDB formation when fed with DDC. Keratin-deficient mice displayed significantly lower numbers of apoptotic hepatocytes than wt animals. krt8⁻/⁻, krt18⁻/⁻ and control mice displayed comparable cell proliferation rates. Chronically DDC-intoxicated krt18⁻/⁻ and wt mice showed a similarly increased degree of steatohepatitis with hepatocyte ballooning and MDB formation. In krt8⁻/⁻ mice, steatosis was less, ballooning, and MDBs were absent. krt18⁻/⁻ mice developed MDBs whereas krt8⁻/⁻ mice on the same genetic background did not, highlighting the significance of different structural properties of keratins. They are independent of the genetic background as an intrinsic factor. By contrast, toxicity effects may depend on the genetic background. krt8⁻/⁻ and krt18⁻/⁻ mice on the same genetic background show similar sensitivity to DDC intoxication and almost resemble wt animals regarding survival, degree of porphyria, liver-to-body weight ratio, serum bilirubin and liver enzyme levels. This stands in contrast to previous work where krt8⁻/⁻ and krt18⁻/⁻ mice on different genetic backgrounds were investigated.
Find related publications in this database (using NLM MeSH Indexing)
Acute Disease -
Animals -
Disease Models, Animal -
Drug-Induced Liver Injury, Chronic - etiology
Fatty Liver - chemically induced
Female -
Gene Expression - drug effects
Genetic Predisposition to Disease -
Keratin-18 - genetics
Keratin-8 - genetics
Liver - drug effects
Male -
Mallory Bodies - drug effects
Mice -
Mice, Knockout -
Organ Size -
Proteins - genetics
Pyridines - toxicity

Find related publications in this database (Keywords)
DDC
intermediate filaments
keratin deficiency
sclerosing cholangitis
steatohepatitis
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