Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

Strobl, H; Scheinecker, C; Riedl, E; Csmarits, B; Bello-Fernandez, C; Pickl, WF; Majdic, O; Knapp, W.
Identification of CD68+lin- peripheral blood cells with dendritic precursor characteristics.
J Immunol. 1998; 161(2):740-748 [OPEN ACCESS]
Web of Science PubMed

 

Autor/innen der Med Uni Graz:
Strobl Herbert
Altmetrics:

Dimensions Citations:

Plum Analytics:
Abstract:
Expression of CD68 (macrosialin) in the absence of surface and lysosomal lineage marker molecules is a characteristic feature of T zone-associated plasmacytoid monocytes, which were recently shown to represent precursors of dendritic cells (DC). We demonstrate here a minor population of strongly CD68-positive (CD68bright) blood cells that lack all analyzed myeloid surface (CD14-, CD33-, CD13-, CD11b-, CD11c-) and lysosomal (myeloperoxidase, MPO- and lysozyme, LZ-) marker molecules (0.4 +/- 2% of the total mononuclear cells). These CD68bright, lineage marker-negative (lin-) cells can be induced to proliferate in the presence of IL-3. They do not acquire myeloid features even upon stimulation with granulocyte-macrophage CSF plus IL-1, IL-3, and IL-6. Instead, these cells develop typical DC characteristics upon culture. Furthermore, these CD68brightlin- DC precursors acquire mature DC characteristics (CD86+, CD83+, CD54bright) upon stimulation with CD40 ligand plus IL-3. A second subset of DC precursor-like blood cells was found to weakly express CD68 (0.3 +/- 0.2% of the total mononuclear cells) and to coexpress several myeloid lineage associated molecules (LZ+, CD11c+, CD33+, CD13+). Cells of this second subset resemble both previously described myeloid-related peripheral blood DC and germinal center DC. Analysis of peripheral blood leukocytes for CD68 thus revealed the existence of two cell subsets that phenotypically resemble lymphoid tissue-associated DC. The unique phenotype CD68brightlin- is highly reminiscent of T zone-associated plasmacytoid monocytes. CD68brightlin- blood leukocytes also functionally resemble plasmacytoid monocytes. The lack of all analyzed myeloid features by CD68brightlin- blood leukocytes suggests that these cells arise from a novel nonmyeloid human DC differentiation pathway.
Find related publications in this database (using NLM MeSH Indexing)
Antigens, CD - biosynthesis
Antigens, CD11 - biosynthesis
Antigens, CD13 - biosynthesis
Antigens, CD34 - biosynthesis
Antigens, CD4 - biosynthesis
Antigens, CD40 - immunology Antigens, CD40 - metabolism
Antigens, CD45 - biosynthesis
Antigens, CD5 - biosynthesis
Antigens, CD7 - biosynthesis
Antigens, Differentiation, Myelomonocytic - biosynthesis
CD40 Ligand -
Cell Differentiation - immunology
Cell Division - immunology
Cell Separation -
Dendritic Cells - cytology Dendritic Cells - immunology Dendritic Cells - metabolism
Granulocytes - cytology
HLA-DR Antigens - biosynthesis
Humans -
Immunoglobulins - biosynthesis
Immunophenotyping -
Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism
Ligands -
Lymphocyte Activation -
Membrane Glycoproteins - biosynthesis Membrane Glycoproteins - immunology Membrane Glycoproteins - metabolism
Monocytes - cytology
Stem Cells - cytology Stem Cells - immunology Stem Cells - metabolism
T-Lymphocytes - immunology
Up-Regulation - immunology

© Meduni Graz Impressum