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Gewählte Publikation:

Hammer, A; Kager, G; Dohr, G; Rabl, H; Ghassempur, I; Jürgens, G.
Generation, characterization, and histochemical application of monoclonal antibodies selectively recognizing oxidatively modified apoB-containing serum lipoproteins.
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Autor/innen der Med Uni Graz:
Dohr Gottfried
Hammer Astrid
Juergens Guenther
Kager Gerd

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Plum Analytics:
To investigate either the role oxidized LDL plays in atherosclerosis or structural changes on the surface of oxidized LDL, monoclonal antibodies (mAbs) are an important tool. After immunizing mice with Cu(2+)-oxidized LDL (oxLDL) and fusion of splenocytes, hybridoma supernatants were screened and cloned. Two mAbs, OB/04 and OB/09 (IgG and IgM), were further characterized. In solid-phase fluorescence immunoassays and Western blot analysis both mAbs reacted with oxLDL, LDL oxidized by a free radical-generating azo compound, or oxVLDL but not with native LDL, acetylated LDL, oxHDL3, azo-oxidized HDL3, or HDL3 modified with malondialdehyde (MDA). In competitive immunoassays with LDL modified by oxidized fatty acid-derived aldehydes, mAb OB/09 strongly reacted with MDA-LDL or MDA-VLDL and LDL modified with 4-hydroxyhexenal followed by 4-hydroxynonenal but not with 4-hydroxyoctenal or hepta-2,4-dienal. mAb OB/04 had a weak affinity for LDL after modification with these aldehydes except for MDA-LDL. LDL modified with arachidonic acid oxidation products (AAOPs) was also recognized by this mAb. However, albumin modified either by the aldehydes applied or by AAOPs did not react with either mAb. Thus, the data indicate that each of the mAbs recognizes a different epitope that is expressed only on apoB-containing lipoproteins upon oxidative modification. An immunostaining with mAb OB/04 was obtained in areas rich in macrophages and in connective tissue of a human atherosclerotic lesion.
Find related publications in this database (using NLM MeSH Indexing)
Amidines - metabolism
Antibodies, Monoclonal - diagnostic use
Antibody Formation - diagnostic use
Antibody Specificity - diagnostic use
Apolipoproteins B - analysis
Arteriosclerosis - metabolism
Blotting, Western - metabolism
Female - metabolism
Humans - metabolism
Hybridomas - immunology
Immunohistochemistry - methods
Lipoproteins - blood
Lipoproteins, LDL - immunology
Male - immunology
Oxidation-Reduction - immunology
Research Support, Non-U.S. Gov't - immunology
Time Factors - immunology

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