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SHR Neuro Krebs Kardio Lipid

Huppertz, B; Kivity, V; Sammar, M; Grimpel, Y; Leepaz, N; Orendi, K; Pekarski, I; Meiri, H; Gonen, R; Lubzens, E.
Cryogenic and low temperature preservation of human placental villous explants - A new way to explore drugs in pregnancy disorders
PLACENTA. 2011; 32: S65-S76.
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Autor/innen der Med Uni Graz:
Aubell Kristina
Huppertz Berthold
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Abstract:
Background: A major handicap in cell culture studies using human tissues is the insufficient availability of fresh material on site. A method was developed for cryogenic storage and low temperature preservation of human placental villous explants, facilitating multi-site distribution for functional studies. Methods: Explants from term placentas were incubated with cryoprotectant agents (dimethyl-sulfoxide (DMSO), ethylene glycol, propanediol or Aedesta), frozen in liquid nitrogen, thawed and then cultured in-vitro. Viability was assessed by comparing frozen and thawed explants with non-frozen controls for morphological changes, lactate dehydrogenase (LDH) release, placenta protein 13 (PP13) secretion, and PCNA Western blotting. Functional studies determined the effect of oxygen and magnesium on explant viability. Results: Cryoprotection by 3 M DMSO best maintained explants' viability, morphological integrity and PP13 release after freezing and thawing from liquid nitrogen. The effect of oxygen and magnesium was used to test the functional viability of cultured explants, after freezing in liquid nitrogen and transfer to dry ice for 1-5 days on site or for shipment to a remote lab. The tested parameters were similar between controls and cryogenically treated explants in the remote lab and the lab of origin, demonstrating the possibility of cryostoring explants for functional studies. Conclusion: Cryogenically stored placental vinous explants shipped frozen can serve as a useful tool for comparative functional studies of placental villous tissues. The results of this pilot study also open the way for multi-site studies associated with drug tailoring for pregnancy disorders. (C) 2010 Elsevier Ltd. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Algorithms -
Cell Survival - drug effects
Cells, Cultured -
Chorionic Villi - drug effects
Cold Temperature -
Cryopreservation - methods
Cryoprotective Agents - pharmacology
Drug Evaluation, Preclinical - methods
Female -
Galectins - metabolism
Humans -
L-Lactate Dehydrogenase - metabolism
Pregnancy -
Pregnancy Complications - drug therapy
Pregnancy Proteins - metabolism
Tissue Preservation - methods
Young Adult -

Find related publications in this database (Keywords)
Cryoprotection
Placenta
Villous explant
PP13
Preeclampsia
DMSO
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