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SHR Neuro Krebs Kardio Lipid

Beraza, N; Ofner-Ziegenfuss, L; Ehedego, H; Boekschoten, M; Bischoff, SC; Mueller, M; Trauner, M; Trautwein, C.
Nor-ursodeoxycholic acid reverses hepatocyte-specific nemo-dependent steatohepatitis.
Gut. 2011; 60(3):387-396
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Autor/innen der Med Uni Graz:
Ofner-Ziegenfuss Lisa
Trauner Michael

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Hepatocyte-specific NEMO/NF-κB deleted mice (NEMO(Δhepa)) develop spontaneous non-alcoholic steatohepatitis (NASH). Free fatty acids and bile acids promote DR5 expression. TRAIL/NK cell-mediated activation of TRAIL-R2/DR5 plays an important role during acute injury in NEMO(Δhepa) mice. To inhibit the progression of NASH in the absence of hepatocyte-NEMO/NF-kB signaling. NEMOf/f and NEMO(Δhepa) mice were fed with a low-fat diet, and with two anticholestatic diets; UDCA and NorUDCA. The impact of these treatments on the progression of NASH was evaluated. We show that high expression of DR5 in livers from NEMO(Δhepa) mice is accompanied by an abundant presence of bile acids (BAs), misregulation of BA transporters and significant alteration of lipid metabolism-related genes. Additionally, mice lacking NEMO in hepatocytes spontaneously showed ductular response at young age. Unexpectedly, feeding of NEMO(Δhepa) mice with low-fat diet failed to improve chronic liver injury. Conversely, anti-cholestatic treatment with nor-ursodeoxycholic acid (NorUDCA), but not with ursodeoxycholic acid (UDCA), led to a significant attenuation of liver damage in NEMO(Δhepa) mice. The strong therapeutic effect of NorUDCA relied on a significant downregulation of LXR-dependent lipogenesis and the normalisation of BA metabolism through mechanisms involving cross-talk between Cyp7a1 and SHP. This was associated with the significant improvement of liver histology, NEMO(Δhepa)/NorUDCA-treated mice showed lower apoptosis and reduced CyclinD1 expression, indicating attenuation of the compensatory proliferative response to hepatocellular damage. Finally, fibrosis and ductular reaction markers were significantly reduced in NorUDCA-treated NEMO(Δhepa) mice. Overall, our work demonstrates the contribution of bile acids metabolism to the progression of NASH in the absence of hepatocyte-NF-kB through mechanisms involving DR5-apoptosis, inflammation and fibrosis. Our work suggests a potential therapeutic effect of NorUDCA in attenuating the progression of NASH.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
Apoptosis - drug effects
Cell Proliferation - drug effects
Diet, Fat-Restricted -
Disease Progression -
Down-Regulation - drug effects
Drug Evaluation, Preclinical -
Fatty Liver - diet therapy
Fatty Liver - drug therapy
Fatty Liver - genetics
Fatty Liver - pathology
Hepatocytes - pathology
Intracellular Signaling Peptides and Proteins - deficiency
Intracellular Signaling Peptides and Proteins - genetics
Intracellular Signaling Peptides and Proteins - physiology
Lipids - biosynthesis
Lipids - genetics
Liver - pathology
Liver Cirrhosis - prevention & control
Mice -
Mice, Knockout -
Oligonucleotide Array Sequence Analysis - methods
Ursodeoxycholic Acid - analogs & derivatives
Ursodeoxycholic Acid - pharmacology
Ursodeoxycholic Acid - therapeutic use

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