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Clifford, N; Smith, LM; Powell, J; Gattenlohner, S; Marx, A; O'Connor, R.
The EphA3 receptor is expressed in a subset of rhabdomyosarcoma cell lines and suppresses cell adhesion and migration.
J Cell Biochem. 2008; 105(5):1250-1259
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Autor/innen der Med Uni Graz:
Gattenlöhner Stefan
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Abstract:
Elevated expression of the Eph receptor tyrosine kinase EphA3 is associated with lymphocytic leukaemia, but little is known about its expression or function in solid tumours. Out of a panel of cancer cell lines, we found that EphA3 was expressed only on two rhabdomyosarcoma (RMS) cell lines of the embryonal histological subtype and on one of the alveolar RMS subtype, whereas it was not detected on two other cell lines of the alveolar subtype. Other EphA receptors (1-7) were, either not expressed in any, or expressed in all five RMS cell lines. Stimulation of EphA3-expressing TE671 and RD RMS cells with ephrinA5 resulted in loss of adhesion to fibronectin, decreased migration towards the stromal cell-derived growth factor-I (SDF-I), increased EphA3 phosphorylation, and increased Rho GTPase activity. In contrast, ectopic expression of EphA3 in the EphA3 negative CRL2061 cell line resulted in decreased cell adhesion. Finally, suppression of EphA3 expression by siRNA in RD cells results in increased SDF-I-mediated motility. These data indicate that EphA3 expression may define subsets of RMS tumours, and that EphA3 suppresses motility through regulation of Rho GTPases in RMS cells.
Find related publications in this database (using NLM MeSH Indexing)
Cell Adhesion - genetics
Cell Line -
Cell Line, Tumor -
Cell Movement -
Ephrin-A5 - metabolism
Fluorescent Antibody Technique -
Humans -
RNA, Small Interfering - metabolism
Receptor, EphA3 - genetics Receptor, EphA3 - metabolism
Rhabdomyosarcoma - genetics Rhabdomyosarcoma - metabolism
Transfection -
rac1 GTP-Binding Protein - metabolism
rho GTP-Binding Proteins - metabolism

Find related publications in this database (Keywords)
EphA3
RHABDOMYOSARCOMA
MIGRATION
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