Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wagner, J.
T-lymphocytes in tendon adhesions and hypertrophic scarring
[ Dissertation ] Medical University of Graz; 2006. pp.
- Autor*innen der Med Uni Graz:
- Betreuer*innen:
- Koch Horst
- Ott Erwin
- Altmetrics:
- Abstract:
- Thea aim of this pilot study was to provide new information of the existence, function, quantity and role of T-lymphocytes in pathological wound healing and scarring by immunihitochemical examination of pathological tendon sheath tissues from patients in comparison to normal tendon sheath tissues form cadavers without scarring. This study involved two groups: the first group included patients (n=6) with posttraumatic tendon adhesions of the hand; biopsy material was taken in the course of tenolysis operations. The exclusion criteria for both groups were: chronic inflammatory disease with activation/inactivation of T-lymphocyte of macrophage-like neoplastic diseases, immunosuppressive treatments and symptoms like morning stiffness and pain and swollen joints, spondylarthritis, rheumatoid arthritis, rheumatic polymyalgia. After staining for immunohystochemistry, the T-lymphocytes were counted manually under a light microscope. The control group (n=6) included cadavers without any diseases or pathological tissue formations of their tendon sheats and without chronic inflammatory disease with T-lymphocyte or macrophage activations as mentioned above. This tendon sheath tissues were taken from the same body part (left of right wrist/palmar hand). Using 6 sample tissues from each group, we analysed and counted the quantity of the immune-flouroescing T-lymphocytes in the histological slices in two ways: first we counted each CD3+ positive cell separately with light microscopy; secondly, we used newly developed computer software for counting the CD3+ positive areas in pixels. The results suggest that T-lymphocytes may play an important regulatory and/or pathological role in wound healing and scar formation because of their extremely high quantities in pathological tissues, up to 15 times higher than in the control group (normal tissues).