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SHR Neuro Krebs Kardio Lipid

Martinez Sanchez, AH; Feyerabend, F; Laipple, D; Willumeit-Römer, R; Weinberg, A; Luthringer, BJC.
Chondrogenic differentiation of ATDC5-cells under the influence of Mg and Mg alloy degradation.
Mater Sci Eng C Mater Biol Appl. 2017; 72(5):378-388
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Autor/innen der Med Uni Graz:
Weinberg Annelie-Martina
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Abstract:
Biodegradable magnesium (Mg)-based materials are a potential alternative to permanent implants for application in children. Nevertheless effects of those materials on growth plate cartilage and chondrogenesis have not been previously evaluated. In vitro differentiation of ATDC5 cells was evaluated under the influence of pure Mg (PMg), Mg with 10wt% of gadolinium (Mg-10Gd) and Mg with 2wt% of silver (Mg-2Ag) degradation products (extracts) and direct cell culture on the materials. Gene expression showed an inhibitory effect on ATDC5 mineralization with the three extracts and a chondrogenic potential of Mg-10Gd. Cells cultured in Mg-10Gd and Mg-2Ag extracts showed the same proliferation and morphology than cells cultured in growth conditions. Mg-10Gd induced an increase in production of ECM and a bigger cell size, similar to the effects found with differentiation conditions. An increased metabolic activity was observed in cells cultured under the influence of Mg-10Gd extracts, indicated by an acidic pH during most of the culture period. After 7days of culture on the materials, ATDC5 growth, distribution and ECM synthesis were higher on Mg-10Gd samples, followed by Mg-2Ag and PMg, which was influenced by the homogeneity and composition of the degradation layer. This study confirmed the tolerance of ATDC5 cells to Mg-based materials and a chondrogenic effect of Mg-10Gd. Further studies in vitro and in vivo are necessary to evaluate cell reactions to those materials, as well as the effects on bone growth and the biocompatibility of the alloying system in the body. Copyright © 2016 Elsevier B.V. All rights reserved.
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Alloys - chemistry
Alloys - metabolism
Alloys - pharmacology
Biocompatible Materials - chemistry
Biocompatible Materials - metabolism
Biocompatible Materials - toxicity
Cell Differentiation - drug effects
Cell Line -
Cell Survival - drug effects
Chondrocytes - cytology
Chondrocytes - metabolism
Chondrogenesis - drug effects
Collagen Type I - genetics
Collagen Type I - metabolism
Core Binding Factor Alpha 1 Subunit - genetics
Core Binding Factor Alpha 1 Subunit - metabolism
Gene Expression - drug effects
Humans -
Hydrogen-Ion Concentration -
Magnesium - chemistry
Microscopy, Electron, Scanning -
SOX9 Transcription Factor - genetics
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Spectrometry, X-Ray Emission -

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Cell differentiation
Chondrogenesis
Biodegradable materials
Magnesium alloys
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