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Coraça-Huber, DC; Wurm, A; Fille, M; Hausdorfer, J; Nogler, M; Kühn, KD.
Effect of freezing on the release rate of gentamicin palmitate and gentamicin sulfate from bone tissue.
J Orthop Res. 2014; 32(6):842-847 [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Kühn Klaus-Dieter
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Abstract:
In this study we evaluated gentamicin palmitate salt and gentamicin sulfate salt mixed with bone chips after storage at -80°C. Different concentration rates of gentamicin sulfate and gentamicin palmitate were mixed with human bone chips and stored for 1-6 months at -80°C. Nonstored samples were used as control. The release of the antibiotics from the bone was carried out in phosphate-buffered saline. Antibiotic concentrations in the elutions were determined with microbiological agar diffusion assay using Bacillus subtilis. Susceptibility tests were carried out using Staphylococci strains. The rate of gentamicin base (GB) released from bone was similar for all gentamicin salts and all storage conditions. The elutions released were efficient on reducing S. aureus and S. epidermidis CFU during all storage time. In resume, the capacity of bone grafts to act as gentamicin carriers has been confirmed in this study. GS + GP showed equivalent efficacy against S. aureus and S. epidermidis compared with GS pure. The lower delivery rate of GS + GP, related to its affinity with fat tissue can be an advantage for longer release times, increasing the local protection against infections. Storage at -80°C does not interfere on the gentamicin salts activity used. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
Find related publications in this database (using NLM MeSH Indexing)
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - metabolism
Anti-Bacterial Agents - pharmacology
Bone Transplantation -
Bone and Bones - metabolism
Coated Materials, Biocompatible - administration & dosage
Freezing -
Gentamicins - administration & dosage
Gentamicins - metabolism
Humans -
Microbial Sensitivity Tests -
Palmitates - administration & dosage
Palmitates - metabolism
Staphylococcal Infections - prevention & control
Staphylococcus aureus - drug effects
Staphylococcus epidermidis - drug effects

Find related publications in this database (Keywords)
bone allografts
storage
anti-infective
gentamicin palmitate
bacillus subtilis
staphylococci
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