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SHR Neuro Krebs Kardio Lipid

Karbiener, M; Pisani, DF; Frontini, A; Oberreiter, LM; Lang, E; Vegiopoulos, A; Mössenböck, K; Bernhardt, GA; Mayr, T; Hildner, F; Grillari, J; Ailhaud, G; Herzig, S; Cinti, S; Amri, EZ; Scheideler, M.
MicroRNA-26 family is required for human adipogenesis and drives characteristics of brown adipocytes.
Stem Cells. 2014; 32(6):1578-1590 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Bernhardt Gerwin
Karbiener Michael
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Abstract:
Adipose tissue contains thermogenic adipocytes (i.e., brown and brite/beige) that oxidize nutrients at exceptionally high rates via nonshivering thermogenesis. Its recent discovery in adult humans has opened up new avenues to fight obesity and related disorders such as diabetes. Here, we identified miR-26a and -26b as key regulators of human white and brite adipocyte differentiation. Both microRNAs are upregulated in early adipogenesis, and their inhibition prevented lipid accumulation while their overexpression accelerated it. Intriguingly, miR-26a significantly induced pathways related to energy dissipation, shifted mitochondrial morphology toward that seen in brown adipocytes, and promoted uncoupled respiration by markedly increasing the hallmark protein of brown fat, uncoupling protein 1. By combining in silico target prediction, transcriptomics, and an RNA interference screen, we identified the sheddase ADAM metallopeptidase domain 17 (ADAM17) as a direct target of miR-26 that mediated the observed effects on white and brite adipogenesis. These results point to a novel, critical role for the miR-26 family and its downstream effector ADAM17 in human adipocyte differentiation by promoting characteristics of energy-dissipating thermogenic adipocytes. © 2013 AlphaMed Press.
Find related publications in this database (using NLM MeSH Indexing)
ADAM Proteins - metabolism
Adipocytes, Brown - cytology Adipocytes, Brown - metabolism Adipocytes, Brown - ultrastructure
Adipogenesis - genetics
Adipose Tissue, White - metabolism Adipose Tissue, White - ultrastructure
Adult -
Cell Differentiation - genetics
Child, Preschool -
Cold Temperature -
Computer Simulation -
Humans -
Infant -
Ion Channels -
Male -
MicroRNAs - genetics MicroRNAs - metabolism
Mitochondria - metabolism Mitochondria - ultrastructure
Mitochondrial Proteins -
Signal Transduction - genetics
Transcriptome - genetics
Up-Regulation - genetics

Find related publications in this database (Keywords)
Adipocyte differentiation
White adipogenesis
Brite adipogenesis
MicroRNA-26
ADAM17
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