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SHR Neuro Krebs Kardio Lipid

Lohberger, B; Stuendl, N; Wolf, E; Liegl-Atzwanger, B; Leithner, A; Rinner, B.
The novel myxofibrosarcoma cell line MUG-Myx1 expresses a tumourigenic stem-like cell population with high aldehyde dehydrogenase 1 activity.
BMC Cancer. 2013; 13(1):563-563 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Leithner Andreas
Liegl-Atzwanger Bernadette
Lohberger Birgit
Rinner Beate
Stündl Nicole
Wolf Elisabeth
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Number of Figures: 3
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Abstract:
Myxofibrosarcoma comprises a spectrum of malignant neoplasms withprominent myxoid stromata, cellular pleomorphism, and distinct curvilinear vascular patterns. These neoplasms mainly affect patients in the sixth to eighth decades of life and the overall 5-year survival rate is 60-70%. After the establishment of the novel myxofibrosarcoma cell lines MUG-Myx1, cells were characterized using short tandem repeat (STR), copy number variation (CNV), and genotype/loss-of-heterozygosity (LOH) analyses. The growth behaviour of the cells was analyzed with the xCELLigence system and an MTS assay. The tumourigenicity of MUG-Myx1 was proved in NOD/SCID mice. Additionally, a stem-like cell population with high enzymatic activity of aldehyde dehydrogenase 1 (ALDH1(high)) was isolated for the first time from myxofibrosarcoma cells using the Aldefluor® assay followed by FACS analysis. The frozen primary parental tumour tissue and the MUG-Myx1 cell line showed the same STR profile at the markers D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Amelogenin, D8S1179, TPOX, and FGY. Typically, myxofibrosarcoma gain and/or amplification was mapped to 7p21.3-q31.1, q31.1-q31.33, q33-q36.2, p21.3, p21.2, p14.1-q11.23, q31.33-q33, p21.2-p14.1, q11.23-q21.3, q36.2-q36.3, which, respectively are known to harbour tumour-associated genes, including TIF, BRAF, MLL3, SMO, and MET. Typically an LOH for myxofibrosarcoma on chr5 q21 was found. In addition, MUG-Myx1 ALDH1(high) cells showed an upregulation of the ABC transporter ABCB1 and ABCG2; higher c-Myc, E-cadherin and SOX-2 expression; and a higher potential for tumourigenicity and proliferation levels. The new myxofibrosarcoma cell line MUG-Myx1 was established to enrich the bank of publicly available cell lines, with respect to providing comprehensive genetic and epigenetic characterization. Furthermore, because of their tumourigenicity, the cell line is also suitable for in vivo experiments.
Find related publications in this database (using NLM MeSH Indexing)
ATP-Binding Cassette Transporters - genetics
Aged -
Animals -
Carcinogenesis - metabolism
Cell Line, Tumor -
Cell Proliferation -
Female -
Fibrosarcoma - enzymology
Gene Expression -
Humans -
Isoenzymes - metabolism
Karyotype -
Loss of Heterozygosity -
Male -
Mice -
Mice, Inbred NOD -
Mice, SCID -
Neoplasm Transplantation -
Neoplastic Stem Cells - enzymology
Retinal Dehydrogenase - metabolism
Thoracic Neoplasms - enzymology

Find related publications in this database (Keywords)
ALDH1
Cancer stem cells
Myxofibrosarcoma
SNP analysis
STR profiling
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