Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Glehr, M; Fritsch-Breisach, M; Lohberger, B; Walzer, SM; Moazedi-Fuerst, F; Rinner, B; Gruber, G; Graninger, W; Leithner, A; Windhager, R.
Influence of resveratrol on rheumatoid fibroblast-like synoviocytes analysed with gene chip transcription.
Phytomedicine. 2013; 20(3-4):310-318
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Breisach Margherita
Glehr Mathias
Graninger Winfried
Gruber Gerald
Leithner Andreas
Lohberger Birgit
Moazedi-Fürst Florentine
Rinner Beate
Windhager Reinhard
Altmetrics:

Dimensions Citations:

Plum Analytics:
Abstract:
Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease that primarily attacks joints and is therefore a common cause of chronic disability and articular destruction. The hyperplastic growth of RA-fibroblast-like synoviocytes (FLSs) and their resistance against apoptosis are considered pathological hallmarks of RA. The natural antioxidant resveratrol is known for its antiproliferative and pro-apoptotic properties. This study investigated the effect of resveratrol on RA-FLS. RA-FLS were isolated from the synovium of 10 RA patients undergoing synovectomy or joint replacement surgery. RA-FLS were first stressed by pre-incubation with interleukin 1beta (IL-1β) and then treated with 100 μM resveratrol for 24h. In order to evaluate the influence of resveratrol on the transcription of genes, a Gene Chip Human Gene 1.0 ST Array was applied. In addition, the effect of dexamethasone on proliferation and apoptosis of RA-FLS was compared with that of resveratrol. Gene array analysis showed highly significant effects of resveratrol on the expression of genes involved in mitosis, cell cycle, chromosome segregation and apoptosis. qRT-PCR, caspase-3/7 and proliferation assays confirmed the results of gene array analysis. In comparison, dexamethasone showed little to no effect on reducing cell proliferation and apoptosis. Our in vitro findings point towards resveratrol as a promising new therapeutic approach for local intra-articular application against RA, and further clinical studies will be necessary. Copyright © 2012 Elsevier GmbH. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Aged -
Anti-Inflammatory Agents - therapeutic use
Antioxidants - pharmacology
Antioxidants - therapeutic use
Apoptosis - drug effects
Arthritis, Rheumatoid - drug therapy
Arthritis, Rheumatoid - genetics
Arthritis, Rheumatoid - metabolism
Caspase 3 - metabolism
Caspase 7 - metabolism
Cell Movement - drug effects
Cell Proliferation - drug effects
Cells, Cultured -
Dexamethasone - therapeutic use
Drug Evaluation, Preclinical -
Female -
Gene Expression Profiling -
Humans -
Male -
Middle Aged -
Oligonucleotide Array Sequence Analysis -
Phenotype -
Phytotherapy -
Real-Time Polymerase Chain Reaction -
Stilbenes - pharmacology
Stilbenes - therapeutic use

Find related publications in this database (Keywords)
Rheumatoid arthritis
Resveratrol
Fibroblast-like synoviocytes
Gene chip transcription analysis
Proliferation
Apoptosis
© Meduni Graz Impressum