Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Lohberger, B; Rinner, B; Stuendl, N; Absenger, M; Liegl-Atzwanger, B; Walzer, SM; Windhager, R; Leithner, A.
Aldehyde dehydrogenase 1, a potential marker for cancer stem cells in human sarcoma.
PLoS One. 2012; 7(8):e43664-e43664 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG


Autor/innen der Med Uni Graz:
Absenger-Novak Markus
Leithner Andreas
Liegl-Atzwanger Bernadette
Lohberger Birgit
Rinner Beate
Stündl Nicole

Dimensions Citations:

Plum Analytics:
Number of Figures: 5
| | | | |
Tumors contain a small population of cancer stem cells (CSC) proposed to be responsible for tumor maintenance and relapse. Aldehyde dehydrogenase 1 (ALDH1) activity has been used as a functional stem cell marker to isolate CSCs in different cancer types. This study used the Aldefluor® assay and fluorescence-activated cell sorting (FACS) analysis to isolate ALDH1(high) cells from five human sarcoma cell lines and one primary chordoma cell line. ALDH1(high) cells range from 0.3% (MUG-Chor1) to 4.1% (SW-1353) of gated cells. Immunohistochemical staining, analysis of the clone formation efficiency, and xCELLigence microelectronic sensor technology revealed that ALDH1(high) cells from all sarcoma cell lines have an increased proliferation rate compared to ALDH1(low) cells. By investigating of important regulators of stem cell biology, real-time RT-PCR data showed an increased expression of c-Myc, β-catenin, and SOX-2 in the ALDH1(high) population and a significant higher level of ABCG2. Statistical analysis of data demonstrated that ALDH1(high) cells of SW-982 and SW-1353 showed higher resistance to commonly used chemotherapeutic agents like doxorubicin, epirubicin, and cisplatin than ALDH1(low) cells. This study demonstrates that in different sarcoma cell lines, high ALDH1 activity can be used to identify a subpopulation of cells characterized by a significantly higher proliferation rate, increased colony forming, increased expression of ABC transporter genes and stemness markers compared to control cells. In addition, enhanced drug resistance was demonstrated.
Find related publications in this database (using NLM MeSH Indexing)
ATP-Binding Cassette Transporters - genetics
Cell Line, Tumor -
Cell Proliferation -
Drug Resistance, Multiple -
Gene Expression Regulation, Neoplastic -
Humans -
Isoenzymes - metabolism
Neoplasm Proteins - genetics
Neoplastic Stem Cells - enzymology
Proto-Oncogene Proteins c-myc - genetics
RNA, Messenger - genetics
Retinal Dehydrogenase - metabolism
SOXB1 Transcription Factors - genetics
Sarcoma - pathology
Tumor Markers, Biological - metabolism
Up-Regulation -
beta Catenin - genetics

© Med Uni Graz Impressum