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SHR Neuro Krebs Kardio Lipid

Pichler, K; Schmidt, B; Fischerauer, EE; Rinner, B; Dohr, G; Leithner, A; Weinberg, AM.
Behaviour of human physeal chondro-progenitorcells in early growth plate injury response in vitro.
Int Orthop. 2012; 36(9):1961-1966 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Amerstorfer Eva Elisa
Dohr Gottfried
Leithner Andreas
Pichler Karin
Rinner Beate
Schmidt Barbara
Weinberg Annelie-Martina
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Number of Figures: 4
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Abstract:
The aim of this study was to investigate the proliferation and differentiation behaviour of a defined cell population gained from the human growth plate, namely, chondro-progenitorcells (CPCs), in the initial inflammatory phase of growth plate injury response in vitro. Growth plate cells were sorted via FACS and differentiated along adipogenic and osteogenic lineage to confirm their progenitor features. To mimic the inflammatory phase of injury response at the growth plate they were treated with IL-1 beta and exposed to cyclic mechanical loading. A BrdU assay was used to investigate CPC proliferation. CPC differentiation behaviour was analysed by RT-PCR. CPCs (CD45-, CD34-, CD73+, CD90+, and CD105+) showed a successful differentiation along adipogenic and osteogenic lineage. Under conditions simulating the inflammatory phase of injury response at the growth plate in vitro CPCs differentiated towards hypertrophy while chondrogenesis and ossification were inhibited. Proliferation was not significantly altered. This study showed that CPCs can be isolated from the human growth plate and expanded in vitro. In the first phase of injury response at the growth plate these cells differentiate towards hypertrophy. As longitudinal growth is obtained by chondrocyte proliferation and volume increase during hypertrophy this maturation might be the first step towards post-traumatic growth disorders such as unwanted premature ossification of the growth plate.
Find related publications in this database (using NLM MeSH Indexing)
Adipocytes - drug effects
Cell Differentiation - drug effects
Cell Enlargement - drug effects
Cell Proliferation - drug effects
Cell Separation -
Cells, Cultured -
Chondrocytes - drug effects
Chondrogenesis - drug effects
Flow Cytometry -
Growth Plate - injuries
Humans -
Interleukin-1beta - pharmacology
Ossification, Heterotopic - chemically induced
Osteocytes - drug effects
Osteogenesis - drug effects
Stem Cells - drug effects
Stress, Mechanical -
Weight-Bearing -

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