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SHR Neuro Krebs Kardio Lipid

Friedmacher, F; Fujiwara, N; Hofmann, AD; Takahashi, H; Alvarez, LA; Gosemann, JH; Puri, P.
Prenatal retinoic acid increases lipofibroblast expression in hypoplastic rat lungs with experimental congenital diaphragmatic hernia.
J Pediatr Surg. 2014; 49(6):876-881
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Autor/innen der Med Uni Graz:
Friedmacher Florian
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Abstract:
Prenatal administration of all-trans retinoic acid (ATRA) has been shown to stimulate alveolarization in nitrofen-induced pulmonary hypoplasia (PH) associated with congenital diaphragmatic hernia (CDH). Lipid-containing interstitial lipofibroblasts (LIFs), characterized by adipocyte differentiation-related protein (ADRP), play a critical role in alveolar development by coordinating lipid homeostasis. Previous studies have demonstrated that ATRA positively affects LIF expression in developing lungs. We hypothesized that pulmonary LIF expression is increased after prenatal ATRA treatment in the nitrofen model of CDH-associated PH. Timed-pregnant rats were treated with nitrofen or vehicle on E9.5, followed by injection of ATRA or placebo on E18.5, E19.5, and E20.5. Fetal lungs were dissected on E21.5 and divided into Control+Placebo, Control+ATRA, Nitrofen+Placebo, and Nitrofen+ATRA. Pulmonary gene expression levels of ADRP were analyzed by quantitative real-time polymerase chain reaction, and LIF expression was investigated by ADRP immunohistochemistry, oil-red-O-, and immunofluorescence-double-staining. Relative mRNA expression of pulmonary ADRP was significantly increased in Nitrofen+ATRA compared to Nitrofen+Placebo (0.31±0.02 vs. 0.08±0.01; P<0.0001). ADRP immunoreactivity and oil-red-O-staining were markedly increased in alveolar interstitium of Nitrofen+ATRA compared to Nitrofen+Placebo. Immunofluorescence-double-staining confirmed markedly increased LIF expression in alveolar walls of Nitrofen+ATRA compared to Nitrofen+Placebo. Increased LIF expression after prenatal treatment with ATRA in nitrofen-induced PH suggests that ATRA may have a therapeutic potential in attenuating CDH-associated PH by stimulating alveolar development. Copyright © 2014 Elsevier Inc. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Cell Differentiation -
Female -
Fibroblasts - drug effects
Fibroblasts - metabolism
Fibroblasts - pathology
Gene Expression Regulation, Developmental - drug effects
Hernias, Diaphragmatic, Congenital - drug therapy
Hernias, Diaphragmatic, Congenital - genetics
Hernias, Diaphragmatic, Congenital - metabolism
Immunohistochemistry -
Lung - abnormalities
Lung - metabolism
Membrane Proteins - biosynthesis
Membrane Proteins - genetics
Organogenesis - drug effects
Perilipin-2 -
Phenyl Ethers - toxicity
Pregnancy -
Pregnancy, Animal -
RNA, Messenger - genetics
Rats -
Rats, Sprague-Dawley -
Real-Time Polymerase Chain Reaction -
Reverse Transcriptase Polymerase Chain Reaction -
Tretinoin - pharmacology
Up-Regulation -

Find related publications in this database (Keywords)
Retinoic acid
Lipofibroblast
Adipocyte differentiation-related protein
Pulmonary hypoplasia
Congenital diaphragmatic hernia
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