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SHR Neuro Krebs Kardio Lipid

Rohban, R; Reinisch, A; Etchart, N; Schallmoser, K; Hofmann, NA; Szoke, K; Brinchmann, JE; Bonyadi Rad, E; Rohde, E; Strunk, D.
Identification of an effective early signaling signature during neo-vasculogenesis in vivo by ex vivo proteomic profiling.
PLoS One. 2013; 8(6):e66909-e66909 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Bonyadirad Ehsan
Hofmann Nicole
Reinisch Andreas
Rohban Rokhsareh
Rohde Eva
Schallmoser Katharina
Strunk Dirk
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Number of Figures: 5
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Abstract:
Therapeutic neo-vasculogenesis in vivo can be achieved by the co-transplantation of human endothelial colony-forming progenitor cells (ECFCs) with mesenchymal stem/progenitor cells (MSPCs). The underlying mechanism is not completely understood thus hampering the development of novel stem cell therapies. We hypothesized that proteomic profiling could be used to retrieve the in vivo signaling signature during the initial phase of human neo-vasculogenesis. ECFCs and MSPCs were therefore either transplanted alone or co-transplanted subcutaneously into immune deficient mice. Early cell signaling, occurring within the first 24 hours in vivo, was analyzed using antibody microarray proteomic profiling. Vessel formation and persistence were verified in parallel transplants for up to 24 weeks. Proteomic analysis revealed significant alteration of regulatory components including caspases, calcium/calmodulin-dependent protein kinase, DNA protein kinase, human ErbB2 receptor-tyrosine kinase as well as mitogen-activated protein kinases. Caspase-4 was selected from array results as one therapeutic candidate for targeting vascular network formation in vitro as well as modulating therapeutic vasculogenesis in vivo. As a proof-of-principle, caspase-4 and general caspase-blocking led to diminished endothelial network formation in vitro and significantly decreased vasculogenesis in vivo. Proteomic profiling ex vivo thus unraveled a signaling signature which can be used for target selection to modulate neo-vasculogenesis in vivo.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Blood Vessels - drug effects
Blood Vessels - growth & development
Blotting, Western -
Caspase Inhibitors - pharmacology
Caspases - metabolism
Endothelial Cells - cytology
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Gene Expression Profiling -
Humans -
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - drug effects
Mesenchymal Stem Cells - metabolism
Mice -
Neovascularization, Physiologic - drug effects
Neovascularization, Physiologic - genetics
Proteomics - methods
Signal Transduction - genetics
Stem Cell Transplantation -

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