Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Singer, G; Stokes, KY; Neil Granger, D.
Reactive oxygen and nitrogen species in sepsis-induced hepatic microvascular dysfunction.
Inflamm Res. 2013; 62(2):155-164 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Singer Georg
Altmetrics:

Dimensions Citations:

Plum Analytics:
Number of Figures: 7
| | | | | | |
Abstract:
Hepatic microvascular dysfunction is a critical event in the development of liver failure during sepsis. Activated blood cells and reactive oxygen and nitrogen species (RONS) have been implicated in the pathogenesis of sepsis. Intravital-videomicroscopy was used to determine whether RONS contribute to the recruitment of leukocytes/platelets in the hepatic microvasculature during sepsis. Six hours following cecal-ligation and puncture (CLP), disturbances of the hepatic microvasculature were assessed in WT-mice (C57Bl/6 J; n = 8), in mice lacking gp91(phox)(n = 5), overexpressing superoxide-dismutase (SOD, n = 8), in WT-mice treated with a NOS-inhibitor (l-NAME, n = 5), lacking nNOS, eNOS or iNOS (n = 5 each), treated with the NO-donor DetaNO (n = 5), in WT-mice treated with gadolinium-chloride (GdCl2, n = 5) and compared to a group of WT-mice following a sham operation (n = 8). Six hours post-CLP, the adhesion of leukocytes and platelets in terminal hepatic venules (THV) and sinusoids was quantified. In WT-mice, CLP elicited increases in the number of adherent leukocytes and platelets. Similar responses to CLP were noted in mice overexpressing SOD or lacking either eNOS or gp91(phox). The blood-cell recruitment was significantly blunted in septic iNOS-knockout mice and this response was reversed by pre-treatment with DetaNO. These findings suggest that iNOS-derived NO is a determinant of the pro-inflammatory phenotype assumed by the hepatic microvasculature during sepsis.
Find related publications in this database (using NLM MeSH Indexing)
Alanine Transaminase - blood
Animals -
Blood Pressure -
Cell Adhesion -
Cell Count -
Cytokines - blood
Gadolinium - pharmacology
Leukocyte Count -
Liver Diseases - etiology Liver Diseases - immunology Liver Diseases - physiopathology
Male -
Membrane Glycoproteins - deficiency Membrane Glycoproteins - genetics
Mice -
Mice, Inbred C57BL -
Mice, Transgenic -
NADPH Oxidase - deficiency NADPH Oxidase - genetics
Nitric Oxide Synthase - deficiency Nitric Oxide Synthase - genetics Nitric Oxide Synthase - immunology
Platelet Count -
Reactive Nitrogen Species - immunology
Reactive Oxygen Species - immunology
Sepsis - complications Sepsis - immunology Sepsis - physiopathology
Superoxide Dismutase - genetics

Find related publications in this database (Keywords)
Nitric oxide
NADPH oxidase
iNOS
eNOS
nNOS
SOD
© Meduni Graz Impressum