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Kofler, K; Ainoedhofer, H; Tausendschon, J; Hollwarth, ME; Saxena, AK.
Esophageal smooth muscle cells dedifferentiate with loss of alpha-smooth muscle actin expression after 8 weeks of explant expansion in vitro culture: Implications on esophagus tissue engineering
EUR SURG. 2011; 43(3): 168-173.
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Autor/innen der Med Uni Graz:
Ainödhofer Herwig
Höllwarth Michael
Kofler Kristina
Saxena Amulya Kumar
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Abstract:
BACKGROUND: Esophagus tissue engineering using the hybrid-construct approach involves assembly of the esophageal components to engineer the organ. This study investigated the dedifferentiation and loss in expression of esophageal smooth muscle after explant expansion in culture. METHODS: Ovine esophagus smooth muscle cells (OESMC) were sourced from adult biopsies and expanded using the explant culture technique. The explants were maintained under static in vitro tissue culture. Flow cytometry analysis was performed for alpha-smooth muscle actin (alpha-SMA) expression at intervals of 4 weeks for up to 8 weeks. RESULTS: After 4 weeks 58.5% OESMC exhibited alpha-SMA which decreased to 28.5% after 6 weeks in culture. After 8 weeks in culture a mere 1.3% OESMC demonstrated alpha-SMA expression. CONCLUSIONS: OESMC proliferated using the explant technique exhibit a total loss of alpha-SMA expression after 8 weeks of static in vitro culture. This information is crucial for tissue engineering of the esophagus using the hybrid-construct approach.

Find related publications in this database (Keywords)
Tissue engineering
esophagus
smooth muscle
flow cytometry
alpha-actin
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