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SHR Neuro Krebs Kardio Lipid

Santigli, E; Leitner, E; Wimmer, G; Kessler, HH; Feierl, G; Grube, M; Eberhard, K; Klug, B.
Accuracy of commercial kits and published primer pairs for the detection of periodontopathogens.
Clin Oral Investig. 2016; 20(9):2515-2528 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Eberhard Katharina
Feierl Gebhard
Kessler Harald
Klug Barbara
Leitner-Meyer Eva
Santigli Elisabeth
Wimmer Gernot
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Abstract:
Despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. The aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens. Fourteen bacterial mock communities, consisting of 16 randomly assembled bacterial strains, were used as reference standard for testing kits and primers. Extracted DNA from mock communities was analyzed by PCR in-house with specific primers and forwarded for analysis to the manufacturer's laboratory of each of the following kits: ParoCheck®Kit 20, micro-IDent®plus11, and Carpegen® Perio Diagnostik. The kits accurately detected Fusobacterium nucleatum, Prevotella intermedia/Prevotella nigrescens, Parvimonas micra, Aggregatibacter actinomycetemcomitans, Campylobacter rectus/showae, Streptococcus mitis, Streptococcus mutans, and Veillonella parvula. The in-house primers for F.nucleatum were highly specific to subtypes of the respective periopathogen. Other primers repeatedly detected oral pathogens not present in the mock communities, indicating reduced specificity. The commercial kits used in this study are reliable tools to support periodontal diagnostics. Whereas the detection profile of the kits is fixed at a general specificity level, the design of primers can be adjusted to differentiate between highly specific strains. In-house primers are more error-prone. Bacterial mock communities can be established as a reference standard for any similar testing. The tested kits render good results with selected bacterial species. Primers appear to be less useful for routine clinical diagnostics and of limited applicability in research. Basic information about the periodontopathogens identified in this study supports clinical decision-making.
Find related publications in this database (using NLM MeSH Indexing)
Bacteriological Techniques -
DNA, Bacterial - analysis
Humans -
Periodontal Diseases - microbiology
Polymerase Chain Reaction -

Find related publications in this database (Keywords)
Aggregatibacter actinomycetemcomitans
Campylobacter rectus/showae
Eikenella corrodens
Fusobacteriumnucleatum
Microbiological diagnosis
Mock community
Molecular diagnostics
Oral biofilm
Parvimonas micra
Periodontal diagnostics
Prevotella intermedia
Prevotella nigrescens
Streptococcusmitis
Streptococcus mutans
Veillonella parvula
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