Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Bagdonaite, I; Wandall, HH; Litvinov, IV; Nastasi, C; Becker, JC; Dabelsteen, S; Geisler, C; Bonefeld, CM; Zhang, Q; Wasik, MA; Zhou, Y; Sasseville, D; Ødum, N; Woetmann, A.
Ectopic expression of a novel CD22 splice-variant regulates survival and proliferation in malignant T cells from cutaneous T cell lymphoma (CTCL) patients.
Oncotarget. 2015; 6(16):14374-14384 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Becker Jürgen Christian
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Number of Figures: 5
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Abstract:
CD22 is a member of the Sialic acid-binding Ig-like lectin (Siglec) family of lectins described to be exclusively present in B lymphocytes and B cell-derived neoplasms. Here, we describe a novel splice form of CD22 (designated CD22∆N), which lacks the N-terminal domain as demonstrated by exon-specific RT-PCR and differential recognition by anti-CD22 antibodies. Importantly, CD22∆N mRNA is expressed in skin lesions from 39 out of 60 patients with cutaneous T cell lymphoma (CTCL), whereas few patients (6 out of 60) expresses full-length, wild type CD22 (CD22wt). In addition, IHC staining of tumor biopsies confirmed the expression of CD22 in CD4+ T cells. Moreover, four out of four malignant T cell lines express CD22: Two cell lines express CD22∆N (MyLa2059 and PB2B) and two express CD22wt (MAC-1 and MAC-2A). siRNA-mediated silencing of CD22 impairs proliferation and survival of malignant T cells, demonstrating a functional role for both CD22∆N and CD22wt in these cells.In conclusion, we provide the first evidence for an ectopic expression of CD22 and a novel splice variant regulating malignant proliferation and survival in CTCL. Analysis of expression and function of CD22 in cutaneous lymphomas may form the basis for development of novel targeted therapies for our patients.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Cell Line, Tumor -
Humans -
Lymphocyte Activation -
Lymphoma, T-Cell, Cutaneous - genetics
Lymphoma, T-Cell, Cutaneous - immunology
Lymphoma, T-Cell, Cutaneous - metabolism
Mice -
Protein Isoforms -
RNA Splicing -
Sialic Acid Binding Ig-like Lectin 2 - biosynthesis
Sialic Acid Binding Ig-like Lectin 2 - genetics
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Transfection -

Find related publications in this database (Keywords)
CTCL
CD22
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