Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Higareda-Almaraz, JC; Karbiener, M; Giroud, M; Pauler, FM; Gerhalter, T; Herzig, S; Scheideler, M.
Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.
BMC Genomics. 2018; 19(1):794-794 [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Karbiener Michael

Dimensions Citations:

Plum Analytics:
Number of Figures: 4
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Norepinephrine (NE) signaling has a key role in white adipose tissue (WAT) functions, including lipolysis, free fatty acid liberation and, under certain conditions, conversion of white into brite (brown-in-white) adipocytes. However, acute effects of NE stimulation have not been described at the transcriptional network level. We used RNA-seq to uncover a broad transcriptional response. The inference of protein-protein and protein-DNA interaction networks allowed us to identify a set of immediate-early genes (IEGs) with high betweenness, validating our approach and suggesting a hierarchical control of transcriptional regulation. In addition, we identified a transcriptional regulatory network with IEGs as master regulators, including HSF1 and NFIL3 as novel NE-induced IEG candidates. Moreover, a functional enrichment analysis and gene clustering into functional modules suggest a crosstalk between metabolic, signaling, and immune responses. Altogether, our network biology approach explores for the first time the immediate-early systems level response of human adipocytes to acute sympathetic activation, thereby providing a first network basis of early cell fate programs and crosstalks between metabolic and transcriptional networks required for proper WAT function.

Find related publications in this database (Keywords)
Norepinephrine stimulation
White adipocyte
Early cell fate
Network biology
Immediate-early gene
Transcriptional regulatory network
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