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Graupp, M; Gruber, HJ; Weiss, G; Kiesler, K; Bachna-Rotter, S; Friedrich, G; Gugatschka, M.
Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria.
Laryngoscope. 2015; 125(6):E203-E209
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Autor/innen der Med Uni Graz:
Bachna-Rotter Sophie
Friedrich Gerhard
Graupp Matthias
Gruber Hans-Jürgen
Gugatschka Markus
Kiesler Karl
Weiss Gregor
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Abstract:
Vocal fold fibrosis represents a major disease burden. Screening of antifibrotic compounds could be facilitated by an in vitro fibrogenesis system. Limitations of existing models might be overcome by implication of the excluded volume effect. In-vitro study. Vocal fold fibroblasts obtained from rats' lamina propria were cultured in four different settings: in standard medium, under "crowded" conditions by adding inert macromolecules, under external administration of transforming growth factor (TGF)ß-1, and under a combination of both. After 5 days, supernatant and cell layer were collected and analyzed by enzyme-linked immunosorbent assay. Immunofluorescence was additionally performed. Collagen-alpha1(I) deposition increased significantly under crowded conditions and after administration of TGFβ-1. Amounts of collagen in the cell layer were significantly higher under crowding conditions with TGFβ-1 compared to administration of TGFβ-1 alone. Crowding enhanced collagen deposition, resulting in more favorable conditions for studying fibrogenesis. This can be the first step toward developing a robust in vitro model for testing antifibrotic compounds. NA. © 2014 The American Laryngological, Rhinological and Otological Society, Inc.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Biomedical Research -
Cells, Cultured -
Collagen - biosynthesis
Extracellular Matrix - metabolism
Fibroblasts -
Fibrosis -
Macromolecular Substances -
Male -
Models, Biological -
Mucous Membrane - pathology
Rats -
Rats, Sprague-Dawley -
Vocal Cords - pathology

Find related publications in this database (Keywords)
Vocal fold scar
in vitro fibrogenesis
fibrosis
macromolecular crowding
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