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SHR Neuro Krebs Kardio Lipid

Mishra, A; Chauhan, G; Violleau, MH; Vojinovic, D; Jian, X; Bis, JC; Li, S; Saba, Y; Grenier-Boley, B; Yang, Q; Bartz, TM; Hofer, E; Soumaré, A; Peng, F; Duperron, MG; Foglio, M; Mosley, TH; Schmidt, R; Psaty, BM; Launer, LJ; Boerwinkle, E; Zhu, Y; Mazoyer, B; Lathrop, M; Bellenguez, C; Van Duijn, CM; Ikram, MA; Schmidt, H; Longstreth, WT; Fornage, M; Seshadri, S; Joutel, A; Tzourio, C; Debette, S.
Association of variants in HTRA1 and NOTCH3 with MRI-defined extremes of cerebral small vessel disease in older subjects.
Brain. 2019; 142(4): 1009-1023. [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Hofer Edith
Saba Yasaman
Schmidt Helena
Schmidt Reinhold
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Abstract:
We report a composite extreme phenotype design using distribution of white matter hyperintensities and brain infarcts in a population-based cohort of older persons for gene-mapping of cerebral small vessel disease. We demonstrate its application in the 3C-Dijon whole exome sequencing (WES) study (n = 1924, nWESextremes = 512), with both single variant and gene-based association tests. We used other population-based cohort studies participating in the CHARGE consortium for replication, using whole exome sequencing (nWES = 2,868, nWESextremes = 956) and genome-wide genotypes (nGW = 9924, nGWextremes = 3308). We restricted our study to candidate genes known to harbour mutations for Mendelian small vessel disease: NOTCH3, HTRA1, COL4A1, COL4A2 and TREX1. We identified significant associations of a common intronic variant in HTRA1, rs2293871 using single variant association testing (Pdiscovery = 8.21 × 10-5, Preplication = 5.25 × 10-3, Pcombined = 4.72 × 10-5) and of NOTCH3 using gene-based tests (Pdiscovery = 1.61 × 10-2, Preplication = 3.99 × 10-2, Pcombined = 5.31 × 10-3). Follow-up analysis identified significant association of rs2293871 with small vessel ischaemic stroke, and two blood expression quantitative trait loci of HTRA1 in linkage disequilibrium. Additionally, we identified two participants in the 3C-Dijon cohort (0.4%) carrying heterozygote genotypes at known pathogenic variants for familial small vessel disease within NOTCH3 and HTRA1. In conclusion, our proof-of-concept study provides strong evidence that using a novel composite MRI-derived phenotype for extremes of small vessel disease can facilitate the identification of genetic variants underlying small vessel disease, both common variants and those with rare and low frequency. The findings demonstrate shared mechanisms and a continuum between genes underlying Mendelian small vessel disease and those contributing to the common, multifactorial form of the disease. © The Author(s) (2019). Published by Oxford University Press on behalf of the Guarantors of Brain.

Find related publications in this database (Keywords)
cerebral small vessel disease
white matter hyperintensity
lacunes of presumed vascular origin
extreme phenotype
exome sequencing study
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