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SHR Neuro Krebs Kardio Lipid

Amplatz, B; Zöhrer, E; Haas, C; Schäffer, M; Stojakovic, T; Jahnel, J; Fauler, G.
Bile acid preparation and comprehensive analysis by high performance liquid chromatography-high-resolution mass spectrometry.
Clin Chim Acta. 2017; 464(2):85-92
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Fauler Günter
Jahnel Jörg
Stojakovic Tatjana
Zöhrer Evelyn
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Abstract:
Accurate analytical methods for bile acid (BA) analysis are important for clinical diagnosis in newborns, adolescents, and adults. Improvements in speed, sensitivity and simplicity enable BA profiling using high performance liquid chromatography (HPLC) together with electrospray ionization (ESI) and high-resolution mass spectrometry (HR-MS). We present a method, validated in different species and tissues, enabling a highly sensitive quantitative determination (in a range of 0.24pmol/sample to 1000pmol/sample, corresponding to 0.024-100pmol on column) of up to 36 naturally occurring BAs from as little as 10μl of plasma or serum, 1ml of urine, or 10mg of dried stool. Chromatographic separation is achieved by HPLC using a C18 reversed phase column and a water/methanol gradient. After ESI, BAs are analyzed through HR-MS using orbitrap technology in full scan mode. 30 different BAs and the corresponding internal standards are separated and analyzed in a single run. Six additional BAs are evaluated in a second run using a pentafluorophenyl (PFP) stationary phase. This method generates detailed human and rodent BA profiles in full scan mode and accurate mass with the advantage of remarkably low required sample volume. Copyright © 2016 Elsevier B.V. All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Adolescent -
Adult -
Analytic Sample Preparation Methods - methods
Bile Acids and Salts - analysis
Bile Acids and Salts - blood
Bile Acids and Salts - isolation & purification
Bile Acids and Salts - urine
Child -
Chromatography, High Pressure Liquid - methods
Clinical Chemistry Tests - methods
Humans -
Infant, Newborn -
Reference Standards -
Reproducibility of Results -
Spectrometry, Mass, Electrospray Ionization - methods

Find related publications in this database (Keywords)
Bile acids
Muricholic acids
High performance liquid chromatography
High resolution-mass spectrometry
Neonates
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