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SHR Neuro Krebs Kardio Lipid

Leis, HJ; Windischhofer, W.
Molecular characterisation of group IVA (cytosolic) phospholipase A2 in murine osteoblastic MC3T3-E1 cells.
Protein J. 2015; 34(1):29-34
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Leis Hans-Jörg
Windischhofer Werner
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Abstract:
Formation of the powerful osteogenic prostaglandin E2 by osteoblasts, a key modulatory event in the paracrine and autocrine regulation of bone cell activity, is preceded by release of the precursor arachidonic acid from phospholipid stores. The main routes of arachidonate liberation may involve phospholipase enzymes such as group IVA phospholipase A2 which is believed to be the main effector in many cell system due to its preference for arachidonate-containing lipids. MC3T3-E1 cells are non-transformed osteoblasts and are widely used as an in vitro model of osteoblast function. In these cells there is still no clarity about the main release pathway of arachidonic acid. Besides cytosolic phospholipase A2, phospholipase C and D pathways may play a key role in arachidonate release. Despite the crucial role of osteoblastic prostgalandin synthesis information on the occurrence of involved enzymes at the molecular level is scarse in MC3T3-E1 cells. We have characterised group IVA phospholipase A2 at the mRNA in these cells as a constitutively expressed enzyme which is cytosolic and translocates to the membrane upon endothelin-1 stimulation. Using immunopurification combined with Western blotting and high-resolution mass spectrometry, the enzyme was also identified at the protein level. Using specific gene silencing we were able to show that osteoblastic cytosolic phospholipase A2 is crucially involved in ET-1-induced prostaglandin formation.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Arachidonic Acid - genetics
Arachidonic Acid - metabolism
Cell Line -
Endothelin-1 - genetics
Endothelin-1 - metabolism
Gene Expression Regulation, Enzymologic - physiology
Group IV Phospholipases A2 - biosynthesis
Group IV Phospholipases A2 - genetics
Mice -
Osteoblasts - cytology
Osteoblasts - enzymology
Prostaglandins - biosynthesis
Prostaglandins - genetics
Protein Transport - physiology

Find related publications in this database (Keywords)
cPLA(2)
Bone
Osteoblast
MC3T3-E1
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