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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Rabensteiner, J; Theiler, G; Duettmann, W; Zollner-Schwetz, I; Hoenigl, M; Valentin, T; Leitner, E; Luxner, J; Grisold, A; Valentin, A; Neumeister, P; Krause, R.
Detection of central venous catheter-related bloodstream infections in haematooncological patients.
Eur J Clin Invest. 2015; 45(8):824-832
Web of Science PubMed FullText FullText_MUG


Autor/innen der Med Uni Graz:
Düttmann Wiebke
Grisold Andrea
Hönigl Martin
Krause Robert
Leitner-Meyer Eva
Luxner Josefa
Neumeister Peter
Rabensteiner Jasmin
Theiler Georg
Valentin Angelika
Valentin Thomas
Zollner-Schwetz Ines

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Plum Analytics:
Catheter-related bloodstream infections (CRBSIs) are currently detected in patients with clinically suspicion. The aim of our study was to evaluate whether CRBSIs could be anticipated and detected in a subclinical stage by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) using universal hybridization probes or acridine orange leucocyte cytospin (AOLC) tests in haematooncological patients with central venous catheters (CVCs) in situ. Peptide nucleic acid fluorescence in situ hybridization and AOLC tests using blood samples from one CVC lumen/port chamber in haematooncological patients were continuously performed. These results were compared to those obtained from routinely performed CRBSI diagnostic tests. One hundred and eighty-two patients with 342 catheter periods were investigated. Seventeen CRBSI cases were detected in 6466 CVC days by routine measures resulting in a CRBSI rate of 2.6/1000 catheter days. Two of 17 showed positive PNA FISH tests, and five positive AOLC test results before the diagnosis were established with routine measures. The screening revealed further seven patients with positive universal PNA FISH tests and 10 positive AOLC tests without symptoms indicative for infection and were therefore considered not to have CRBSI. Sampling of only one CVC lumen/port chamber screening for CRBSI in haematooncological patients seems not to be a useful tool for anticipative diagnosis of CRBSI. Reasons for false-negative results might include origin of CRBSIs from the other CVC lumina not sampled for screening, and false-positive results might origin from catheter colonization without subsequent spread of micro-organisms into the peripheral bloodstream. © 2015 Stichting European Society for Clinical Investigation Journal Foundation.
Find related publications in this database (using NLM MeSH Indexing)
Acridine Orange -
Adult -
Aged -
Bacteremia - complications
Bacteremia - diagnosis
Candidiasis - complications
Candidiasis - diagnosis
Catheter-Related Infections - complications
Catheter-Related Infections - diagnosis
Central Venous Catheters -
Cohort Studies -
Enterobacteriaceae Infections - complications
Enterobacteriaceae Infections - diagnosis
Escherichia coli Infections - complications
Escherichia coli Infections - diagnosis
False Negative Reactions -
Female -
Fluorescent Dyes -
Fungemia - complications
Fungemia - diagnosis
Gram-Negative Bacterial Infections - complications
Gram-Negative Bacterial Infections - diagnosis
Gram-Positive Bacterial Infections - complications
Gram-Positive Bacterial Infections - diagnosis
Hematologic Neoplasms - complications
Humans -
In Situ Hybridization, Fluorescence -
Klebsiella Infections - complications
Klebsiella Infections - diagnosis
Male -
Middle Aged -
Peptide Nucleic Acids -
Pseudomonas Infections - complications
Pseudomonas Infections - diagnosis
Staphylococcal Infections - complications
Staphylococcal Infections - diagnosis
Stenotrophomonas maltophilia -

Find related publications in this database (Keywords)
Acridine orange leucocyte cytospin
catheter-related blood stream infection
peptide nucleic acid fluorescence insitu hybridization
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