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SHR Neuro Krebs Kardio Lipid

Fink, SE; Gandhi, MK; Nourse, JP; Keane, C; Jones, K; Crooks, P; Jöhrens, K; Korfel, A; Schmidt, H; Neumann, S; Tiede, A; Jäger, U; Dührsen, U; Neuhaus, R; Dreyling, M; Borchert, K; Südhoff, T; Riess, H; Anagnostopoulos, I; Trappe, RU.
A comprehensive analysis of the cellular and EBV-specific microRNAome in primary CNS PTLD identifies different patterns among EBV-associated tumors.
Am J Transplant. 2014; 14(11):2577-2587 [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Schmidt Helmut
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Abstract:
Primary central nervous system (pCNS) posttransplant lymphoproliferative disorder (PTLD) is a complication of solid organ transplantation characterized by poor outcome. In contrast to systemic PTLD, Epstein-Barr virus (EBV)-association of pCNS PTLD is almost universal, yet viral and cellular data are limited. To identify differences in the pattern of EBV-association of pCNS and systemic PTLD, we analyzed the expression of latent and lytic EBV transcripts and the viral and cellular microRNAome in nine pCNS (eight EBV-associated) and in 16 systemic PTLD samples (eight EBV-associated). Notably although 15/16 EBV-associated samples exhibited a viral type III latency pattern, lytic transcripts were also strongly expressed. Members of the ebv-miR-BHRF1 and ebv-miR-BART clusters were expressed in virtually all EBV-associated PTLD samples. There were 28 cellular microRNAs differentially expressed between systemic and pCNS PTLD. pCNS PTLD expressed lower hsa-miR-199a-5p/3p and hsa-miR-143/145 (implicated in nuclear factor kappa beta and c-myc signaling) as compared to systemic PTLD. Unsupervised nonhierarchical clustering of the viral and cellular microRNAome distinguished non-EBV-associated from EBV-associated samples and identified a separate group of EBV-associated pCNS PTLD that displayed reduced levels of B cell lymphoma associated oncomiRs such as hsa-miR-155, -21, -221 and the hsa-miR-17-92 cluster. EBV has a major impact on viral and cellular microRNA expression in EBV-associated pCNS PTLD. © Copyright 2014 The American Society of Transplantation and the American Society of Transplant Surgeons.
Find related publications in this database (using NLM MeSH Indexing)
Cell Line, Transformed -
Central Nervous System Neoplasms - genetics
Central Nervous System Neoplasms - virology
Female -
Gene Expression Profiling -
Herpesvirus 4, Human - genetics
Humans -
Lymphoproliferative Disorders - genetics
Lymphoproliferative Disorders - virology
Male -
MicroRNAs - genetics
Transcriptome -

Find related publications in this database (Keywords)
Kidney transplantation
nephrology
molecular biology: micro RNA
posttransplant lymphoproliferative disorder (PTLD)
translational research
science
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