Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Logo MUG-Forschungsportal

Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Zebisch, A; Wölfler, A; Fried, I; Wolf, O; Lind, K; Bodner, C; Haller, M; Drasche, A; Pirkebner, D; Matallanas, D; Rath, O; Blyth, K; Delwel, R; Taskesen, E; Quehenberger, F; Kolch, W; Troppmair, J; Sill, H.
Frequent loss of RAF kinase inhibitor protein expression in acute myeloid leukemia.
Leukemia. 2012; 26(8):1842-1849 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Bodner Claudia
Fried Isabella
Lind Karin
Quehenberger Franz
Sill Heinz
Wölfler Albert
Zebisch Armin
Altmetrics:

Dimensions Citations:

Plum Analytics:
Abstract:
RAF kinase inhibitor protein (RKIP) is a negative regulator of the RAS-mitogen-activated protein kinase/extracellular signal-regulated kinase signaling cascade. We investigated its role in acute myeloid leukemia (AML), an aggressive malignancy arising from hematopoietic stem and progenitor cells (HSPCs). Western blot analysis revealed loss of RKIP expression in 19/103 (18%) primary AML samples and 4/17 (24%) AML cell lines but not in 10 CD34+ HSPC specimens. In in-vitro experiments with myeloid cell lines, RKIP overexpression inhibited cellular proliferation and colony formation in soft agar. Analysis of two cohorts with 103 and 285 AML patients, respectively, established a correlation of decreased RKIP expression with monocytic phenotypes. RKIP loss was associated with RAS mutations and in transformation assays, RKIP decreased the oncogenic potential of mutant RAS. Loss of RKIP further related to a significantly longer relapse-free survival and overall survival in uni- and multivariate analyses. Our data show that RKIP is frequently lost in AML and correlates with monocytic phenotypes and mutations in RAS. RKIP inhibits proliferation and transformation of myeloid cells and decreases transformation induced by mutant RAS. Finally, loss of RKIP seems to be a favorable prognostic parameter in patients with AML.
Find related publications in this database (using NLM MeSH Indexing)
Cell Differentiation - genetics
Cell Line, Tumor -
Cell Proliferation -
Cell Transformation, Neoplastic - genetics
Gene Expression Regulation, Leukemic -
Genes, ras -
Humans -
Leukemia, Myeloid, Acute - genetics Leukemia, Myeloid, Acute - metabolism Leukemia, Myeloid, Acute - mortality
Monocytes - cytology Monocytes - metabolism
Mutation -
Myeloid Cells - metabolism
Phosphatidylethanolamine Binding Protein - deficiency Phosphatidylethanolamine Binding Protein - genetics Phosphatidylethanolamine Binding Protein - metabolism
Prognosis -

Find related publications in this database (Keywords)
RAF kinase inhibitor protein
acute myeloid leukemia
RAS mutation
© Meduni Graz Impressum