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SHR Neuro Krebs Kardio Lipid

Flemming, A; Schallmoser, K; Strunk, D; Stolk, M; Volk, HD; Seifert, M.
Immunomodulative efficacy of bone marrow-derived mesenchymal stem cells cultured in human platelet lysate.
J Clin Immunol. 2011; 31(6):1143-1156
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Autor/innen der Med Uni Graz:
Schallmoser Katharina
Strunk Dirk
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Abstract:
Human mesenchymal stem cells (hMSCs) are considered to be a promising tool for novel cell-based therapies. Clinical applications in solid organ transplantation were hampered by the dependence on animal serum for hMSCs clinical scale expansion until substitution with human platelet lysate (HPL) became a promising alternative. Therefore we focused on a direct comparison of immunomodulatory properties of hMSCs cultured in HPL or fetal calf serum (FCS). Phenotypic characterization, detection of cytokine secretion and effects on alloantigen- and mitogen-induced lymphocyte proliferation as well as degranulation of cytomegalovirus-specific cytotoxic T cells were applied in potency assays. We demonstrated that HPL-cultured MSCs have comparable immunomodulatory capacities to their FCS-cultured counterparts. The observed immunomodulatory properties include a beneficial inhibitory effect on immune cell proliferation and an unaffected viral T cell immunity. Thus, culturing hMSCs in HPL generates an efficient and safe expansion combined with intriguing immunomodulatory properties making these cells an attractive cell therapeutic tool.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Antigens, Viral - immunology
Blood Platelets - metabolism
Bone Marrow - pathology
Cattle -
Cell Extracts - immunology
Cell Proliferation -
Cells, Cultured -
Culture Media - metabolism
Cytomegalovirus - immunology
Cytotoxicity, Immunologic -
Humans -
Immunomodulation -
Isoantigens - immunology
Mesenchymal Stem Cell Transplantation -
Mesenchymal Stem Cells - immunology
Serum - immunology
T-Lymphocytes, Cytotoxic - immunology

Find related publications in this database (Keywords)
Immunomodulation
mesenchymal stem cell
human platelet lysate
cell therapy
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