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SHR Neuro Krebs Kardio Lipid

Mayr, C; Wagner, A; Stoecklinger, A; Jakab, M; Illig, R; Berr, F; Pichler, M; Di Fazio, P; Ocker, M; Neureiter, D; Kiesslich, T.
3-Deazaneplanocin A May Directly Target Putative Cancer Stem Cells in Biliary Tract Cancer.
Anticancer Res. 2015; 35(9):4697-4705
Web of Science PubMed

 

Autor/innen der Med Uni Graz:
Pichler Martin
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Abstract:
Polycomb repressive complex 2 (PRC2), an epigenetic master regulator, contributes to progression and development of biliary tract cancer (BTC). The present study investigated the effects of the PRC2 inhibitor 3-deazaneplanocin A (DZNep) on BTC cell lines. In vitro effects of DZNep treatment were analyzed for cell viability, gene expression and functional characteristics of cancer stem cell (CSC). DZNep treatment caused a cell line- and dose-dependent decrease in viability. In the EGI-1 cell line, a direct cytotoxic effect was accompanied by mRNA down-regulation of the PRC2 core components, cyclins as well as of CSC-related genes. Furthermore, DZNep affected putative CSCs by reduction of sphere formation and aldehyde dehydrogenase-1-positive cells. The stem cell characteristics of these subpopulations were verified by real-time polymerase chain reaction analysis. Taken together, our results show that DZNep might be a promising pharmacological agent for future therapies regarding BTC. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
Find related publications in this database (using NLM MeSH Indexing)
Adenosine - analogs & derivatives
Adenosine - pharmacology
Biliary Tract Neoplasms - genetics
Biliary Tract Neoplasms - pathology
Biomarkers, Tumor - metabolism
Cell Death - drug effects
Cell Line, Tumor -
Cell Survival - drug effects
Cyclins - genetics
Cyclins - metabolism
Dose-Response Relationship, Drug -
Gene Expression Regulation, Neoplastic - drug effects
Humans -
Neoplastic Stem Cells - drug effects
Neoplastic Stem Cells - metabolism
Neoplastic Stem Cells - pathology
Polycomb Repressive Complex 2 - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism

Find related publications in this database (Keywords)
Biliary tract cancer
EZH2
PRC2
DZNep
anchorage-independent growth
cancer stem cells
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