Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Bode, GH; Pickl, KE; Sanchez-Purrà, M; Albaiges, B; Borrós, S; Pötgens, AJ; Schmitz, C; Sinner, FM; Losen, M; Steinbusch, HW; Frank, HG; Martinez-Martinez, P; European NanoBioPharmaceutics Research Initiative.
Detection of Peptide-based nanoparticles in blood plasma by ELISA.
PLoS One. 2015; 10(5):e0126136-e0126136 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Sinner Frank
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Number of Figures: 4
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Abstract:
The aim of the current study was to develop a method to detect peptide-linked nanoparticles in blood plasma. A convenient enzyme linked immunosorbent assay (ELISA) was developed for the detection of peptides functionalized with biotin and fluorescein groups. As a proof of principle, polymerized pentafluorophenyl methacrylate nanoparticles linked to biotin-carboxyfluorescein labeled peptides were intravenously injected in Wistar rats. Serial blood plasma samples were analyzed by ELISA and by liquid chromatography mass spectrometry (LC/MS) technology. The ELISA based method for the detection of FITC labeled peptides had a detection limit of 1 ng/mL. We were able to accurately measure peptides bound to pentafluorophenyl methacrylate nanoparticles in blood plasma of rats, and similar results were obtained by LC/MS. We detected FITC-labeled peptides on pentafluorophenyl methacrylate nanoparticles after injection in vivo. This method can be extended to detect nanoparticles with different chemical compositions.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Chromatography, Liquid -
Enzyme-Linked Immunosorbent Assay - methods
Male -
Nanoparticles -
Peptides - blood
Plasma -
Rats -
Tandem Mass Spectrometry -

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