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SHR Neuro Krebs Kardio Lipid

Schwarzenbacher, D; Stiegelbauer, V; Deutsch, A; Ress, AL; Aigelsreiter, A; Schauer, S; Wagner, K; Langsenlehner, T; Resel, M; Gerger, A; Ling, H; Ivan, C; Calin, GA; Hoefler, G; Rinner, B; Pichler, M.
Low spinophilin expression enhances aggressive biological behavior of breast cancer.
Oncotarget. 2015; 6(13):11191-11202 [OPEN ACCESS]
Web of Science PubMed PUBMED Central FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Aigelsreiter Ariane
Deutsch Alexander
Gerger Armin
Hoefler Gerald
Langsenlehner Tanja
Lembeck Anna Lena
Pichler Martin
Resel Margit
Rinner Beate
Schauer Silvia
Schwarzenbacher Daniela
Stiegelbauer Verena
Wagner Karin
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Number of Figures: 4
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Abstract:
Spinophilin, a putative tumor suppressor gene, has been shown to be involved in the pathogenesis of certain types of cancer, but its role has never been systematically explored in breast cancer. In this study, we determined for the first time the expression pattern of spinophilin in human breast cancer molecular subtypes (n = 489) and correlated it with survival (n = 921). We stably reduced spinophilin expression in breast cancer cells and measured effects on cellular growth, apoptosis, anchorage-independent growth, migration, invasion and self-renewal capacity in vitro and metastases formation in vivo. Microarray profiling was used to determine the most abundantly expressed genes in spinophilin-silenced breast cancer cells. Spinophilin expression was significantly lower in basal-like breast cancer (p<0.001) and an independent poor prognostic factor in breast cancer patients (hazard ratio = 1.93, 95% confidence interval: 1.24 -3.03; p = 0.004) A reduction of spinophilin levels increased cellular growth in breast cancer cells (p<0.05), without influencing activation of apoptosis. Anchorage-independent growth, migration and self-renewal capacity in vitro and metastatic potential in vivo were also significantly increased in spinophilin-silenced cells (p<0.05). Finally, we identified several differentially expressed genes in spinophilin-silenced cells. According to our data, low levels of spinophilin are associated with aggressive behavior of breast cancer.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Apoptosis -
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Blotting, Western -
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Breast Neoplasms - mortality
Breast Neoplasms - pathology
Cell Movement -
Cell Proliferation -
Cell Transformation, Neoplastic -
Female -
Gene Expression Profiling -
Gene Expression Regulation, Neoplastic -
Humans -
Immunoenzyme Techniques -
Mice -
Mice, Inbred NOD -
Mice, SCID -
Microfilament Proteins - genetics
Microfilament Proteins - metabolism
Neoplasm Staging -
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Prognosis -
RNA, Messenger - genetics
Real-Time Polymerase Chain Reaction -
Reverse Transcriptase Polymerase Chain Reaction -
Survival Rate -
Tumor Cells, Cultured -
Xenograft Model Antitumor Assays -

Find related publications in this database (Keywords)
breast cancer
tumor suppressor
prognosis
cellular growth
invasion
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