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SHR Neuro Krebs Kardio Lipid

Sendlhofer, G; Brunner, G; Schaupp, L; Wutte, A; Ellmerer, M; Pieber, TR.
Estimation of human leptin concentration in the subcutaneous adipose and skeletal muscle tissues.
Eur J Clin Invest. 2015; 45(5):445-451 [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Brunner Gernot
Ellmerer Martin
Pieber Thomas
Schaupp Lukas
Sendlhofer Gerald
Wutte Andrea Maria
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Abstract:
Interstitial leptin concentrations in subcutaneous adipose and skeletal muscle tissues were determined by open-flow microperfusion. In 12 lean male subjects (age: 25.6 ± 1.1 years), a zero flow rate experiment using different flow rates was applied. Recovery was determined by urea as an internal reference. In the no-net-flux experiments, catheters were perfused with five solutions containing different concentrations of leptin. Concentrations of interstitial leptin were calculated by applying linear regression analysis to perfusate as opposed to sampled leptin concentrations. The zero flow rate protocol showed significantly higher concentrations of leptin in the interstitial fluid of subcutaneous adipose compared to skeletal muscle tissue [36.8 ± 10.32 vs. 7.1 ± 2.5% of the corresponding plasma level (P = 0.018)]. The recovery of urea in the samples was comparable for all catheters [79.4 ± 6.8 vs. 83.0 ± 5.8 of the corresponding plasma level, flow rate of 0.3 μL/min; (P = ns)] and was higher when compared to leptin. In the no-net-flux protocol, the concentration of leptin in subcutaneous adipose tissue was almost identical to plasma [90. 5 ± 7.0%] and the skeletal muscle tissue concentration of leptin was 23.7 ± 2.5% of the corresponding plasma level. Open-flow microperfusion enables the estimation of leptin concentrations in subcutaneous adipose and skeletal muscle tissues in humans in vivo. This is the first documentation on the use of open-flow microperfusion to demonstrate that relevant amounts of leptin are also found in skeletal muscle tissue. © 2015 Stichting European Society for Clinical Investigation Journal Foundation.
Find related publications in this database (using NLM MeSH Indexing)
Adult -
Humans -
Leptin - metabolism
Linear Models -
Male -
Muscle, Skeletal - metabolism
Subcutaneous Fat - metabolism
Urea - metabolism
Young Adult -

Find related publications in this database (Keywords)
adipose tissue
invivo
interstitial fluid
leptin
skeletal muscle
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