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SHR Neuro Krebs Kardio Lipid

Diwoky, C; Liebmann, D; Neumayer, B; Reinisch, A; Knoll, F; Strunk, D; Stollberger, R.
Positive contrast of SPIO-labeled cells by off-resonant reconstruction of 3D radial half-echo bSSFP.
NMR BIOMED. 2015; 28(1): 79-88.
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Autor/innen der Med Uni Graz:
Diwoky Clemens
Neumayer Bernhard
Reinisch Andreas
Stollberger Rudolf
Strunk Dirk
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Abstract:
This article describes a new acquisition and reconstruction concept for positive contrast imaging of cells labeled with superparamagnetic iron oxides (SPIOs). Overcoming the limitations of a negative contrast representation as gained with gradient echo and fully balanced steady state (bSSFP), the proposed method delivers a spatially localized contrast with high cellular sensitivity not accomplished by other positive contrast methods. Employing a 3D radial bSSFP pulse sequence with half-echo sampling, positive cellular contrast is gained by adding artificial global frequency offsets to each half-echo before image reconstruction. The new contrast regime is highlighted with numerical intravoxel simulations including the point-spread function for 3D half-echo acquisitions. Furthermore, the new method is validated on the basis of in vitro cell phantom measurements on a clinical MRI platform, where the measured contrast-to-noise ratio (CNR) of the new approach exceeds even the negative contrast of bSSFP. Finally, an in vivo proof of principle study based on a mouse model with a clear depiction of labeled cells within a subcutaneous cell islet containing a cell density as low as 7 cells/mm(3) is presented. The resultant isotropic images show robustness to motion and a high CNR, in addition to an enhanced specificity due to the positive contrast of SPIO-labeled cells. Copyright © 2014 John Wiley & Sons, Ltd.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Computer Simulation -
Contrast Media -
Dextrans - metabolism
Humans -
Imaging, Three-Dimensional -
Injections, Subcutaneous -
Magnetic Resonance Imaging - methods
Magnetite Nanoparticles -
Mice -
Phantoms, Imaging -
Signal Processing, Computer-Assisted -
Staining and Labeling -

Find related publications in this database (Keywords)
stem cell tracking
positive contrast
off-resonant reconstruction
bSSFP
3D radial half-echo sampling
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