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SHR Neuro Krebs Kardio Lipid

Bernhart, E; Kollroser, M; Rechberger, G; Reicher, H; Heinemann, A; Schratl, P; Hallström, S; Wintersperger, A; Nusshold, C; Devaney, T; Zorn-Pauly, K; Malli, R; Graier, W; Malle, E; Sattler, W.
Lysophosphatidic acid receptor activation affects the C13NJ microglia cell line proteome leading to alterations in glycolysis, motility, and cytoskeletal architecture.
Proteomics. 2010; 10(1): 141-158. [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Bernhart Eva Maria
Devaney Trevor
Graier Wolfgang
Hallström Seth
Heinemann Akos
Kollroser Manfred
Luschnig Petra
Malle Ernst
Malli Roland
Nusshold Christoph
Sattler Wolfgang
Wintersperger Andrea
Zorn-Pauly Klaus
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Number of Figures: 9
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Abstract:
Microglia, the immunocompetent cells of the CNS, are rapidly activated in response to injury and microglia migration towards and homing at damaged tissue plays a key role in CNS regeneration. Lysophosphatidic acid (LPA) is involved in signaling events evoking microglia responses through cognate G protein-coupled receptors. Here we show that human immortalized C13NJ microglia express LPA receptor subtypes LPA(1), LPA(2), and LPA(3) on mRNA and protein level. LPA activation of C13NJ cells induced Rho and extracellular signal-regulated kinase activation and enhanced cellular ATP production. In addition, LPA induced process retraction, cell spreading, led to pronounced changes of the actin cytoskeleton and reduced cell motility, which could be reversed by inhibition of Rho activity. To get an indication about LPA-induced global alterations in protein expression patterns a 2-D DIGE/LC-ESI-MS proteomic approach was applied. On the proteome level the most prominent changes in response to LPA were observed for glycolytic enzymes and proteins regulating cell motility and/or cytoskeletal dynamics. The present findings suggest that naturally occurring LPA is a potent regulator of microglia biology. This might be of particular relevance in the pathophysiological context of neurodegenerative disorders where LPA concentrations can be significantly elevated in the CNS.
Find related publications in this database (using NLM MeSH Indexing)
Cell Line -
Cell Movement -
Cytoskeleton - metabolism
Gene Expression Regulation -
Glycolysis -
Humans -
Lysophospholipids - metabolism
Microglia - cytology Microglia - metabolism
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3 - metabolism
Proteome - metabolism
Receptors, Lysophosphatidic Acid - genetics Receptors, Lysophosphatidic Acid - metabolism
Signal Transduction -
rho GTP-Binding Proteins - metabolism

Find related publications in this database (Keywords)
2-D DIGE
Cell biology
Lipids
Migration
MS
Rho
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