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SHR Neuro Krebs Kardio Lipid

Chantepie, S; Malle, E; Sattler, W; Chapman, MJ; Kontush, A.
Distinct HDL subclasses present similar intrinsic susceptibility to oxidation by HOCl.
Arch Biochem Biophys. 2009; 487(1): 28-35. [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Malle Ernst
Sattler Wolfgang
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Number of Figures: 6
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Abstract:
The heme protein myeloperoxidase (MPO) functions as a catalyst for lipoprotein oxidation. Hypochlorous acid (HOCl), a potent two-electron oxidant formed by the MPO-H(2)O(2)-chloride system of activated phagocytes, modifies antiatherogenic high-density lipoprotein (HDL). The structural heterogeneity and oxidative susceptibility of HDL particle subfractions were probed with HOCl. All distinct five HDL subfraction were modified by HOCl as demonstrated by the consumption of tryptophan residues and free amino groups, cross-linking of apolipoprotein AI, formation of HOCl-modified epitopes, increased electrophoretic mobility and altered content of unsaturated fatty acids in HDL subclasses. Small, dense HDL3 were less susceptible to oxidative modification than large, light HDL2 on a total mass basis at a fixed HOCl:HDL mass ratio of 1:32, but in contrast not on a particle number basis at a fixed HOCl:HDL molar ratio of 97:1. We conclude that structural and physicochemical differences between HDL subclasses do not influence their intrinsic susceptibility to oxidative attack by HOCl.
Find related publications in this database (using NLM MeSH Indexing)
Atherosclerosis - blood Atherosclerosis - etiology
Blood Protein Electrophoresis -
Electrophoresis, Agar Gel -
Fatty Acids, Unsaturated - analysis
Humans -
Hypochlorous Acid - pharmacology
Lipoproteins, HDL - blood Lipoproteins, HDL - chemistry Lipoproteins, HDL - classification Lipoproteins, HDL - drug effects
Lipoproteins, HDL2 - blood Lipoproteins, HDL2 - chemistry Lipoproteins, HDL2 - drug effects
Lipoproteins, HDL3 - blood Lipoproteins, HDL3 - chemistry Lipoproteins, HDL3 - drug effects
Oxidants - pharmacology
Oxidation-Reduction -
Tryptophan - chemistry

Find related publications in this database (Keywords)
Antiatherogenic lipoprotein
Hypochlorite
Myeloperoxidase-hydrogen peroxide-halide system
Tryptophan residues
Lysine modification
Apolipoprotein Al
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