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SHR Neuro Krebs Kardio Lipid

Kratzer, I; Bernhart, E; Wintersperger, A; Hammer, A; Waltl, S; Malle, E; Sperk, G; Wietzorrek, G; Dieplinger, H; Sattler, W.
Afamin is synthesized by cerebrovascular endothelial cells and mediates alpha-tocopherol transport across an in vitro model of the blood-brain barrier.
J Neurochem. 2009; 108(3): 707-718. [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Bernhart Eva Maria
Hammer Astrid
Kratzer Ingrid
Malle Ernst
Sattler Wolfgang
Waltl Sabine Evelyn
Wintersperger Andrea

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Number of Figures: 7
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Alpha-tocopherol (alphaTocH), a member of the vitamin E family, is essential for normal neurological function. Despite the importance of alphaTocH transport into the CNS, transfer mechanisms across the blood-brain barrier (BBB) are not entirely clear. We here investigate whether afamin, a known alphaTocH-binding protein, contributes to alphaTocH transport across an in vitro model of the BBB consisting of primary porcine brain capillary endothelial cells (BCEC) and basolaterally cultured astrocytoma cells. Exogenously added afamin had no adverse effects on BCEC viability or barrier function and was transported across BCEC Transwell cultures. Furthermore, alphaTocH transport across polarized BCEC cultures to astrocytoma cells is facilitated by afamin, though to a lesser extent than by high-density lipoprotein-mediated transport, an essential and in vivo operating alphaTocH import pathway at the cerebrovasculature. We also demonstrate that porcine BCEC endogenously synthesize afamin. In line with these in vitro findings, afamin was detected by immunohistochemistry in porcine, human postmortem, and mouse brain, where prominent staining was observed almost exclusively in the cerebrovasculature. The demonstration of afamin mRNA expression in isolated brain capillaries suggests that afamin might be a new family member of binding/transport proteins contributing to alphaTocH homeostasis at the BBB in vivo.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Astrocytes - drug effects
Astrocytoma - metabolism
Biological Transport, Active -
Blood-Brain Barrier - physiology
Blotting, Western -
CHO Cells -
Capillaries - metabolism
Carrier Proteins - biosynthesis
Cerebrovascular Circulation - physiology
Coculture Techniques -
Cricetinae -
Cricetulus -
Electrophoresis, Polyacrylamide Gel -
Endothelial Cells - metabolism
Fluorescent Antibody Technique -
Glycoproteins - biosynthesis
Humans -
Lipoproteins, HDL - biosynthesis
Mice -
Mice, Inbred C57BL -
RNA - biosynthesis
Recombinant Proteins - biosynthesis
Serum Albumin - biosynthesis
Swine -
Tetrazolium Salts -
Thiazoles -
alpha-Tocopherol - metabolism

Find related publications in this database (Keywords)
brain microvascular endothelial cells
high-density lipoprotein
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