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Gewählte Publikation:

SHR Neuro Krebs Kardio Lipid

Kovacevic, A; Hammer, A; Stadelmeyer, E; Windischhofer, W; Sundl, M; Ray, A; Schweighofer, N; Friedl, G; Windhager, R; Sattler, W; Malle, E.
Expression of serum amyloid A transcripts in human bone tissues, differentiated osteoblast-like stem cells and human osteosarcoma cell lines.
J Cell Biochem. 2008; 103(3): 994-1004. [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Friedl Gerald
Hammer Astrid
Malle Ernst
Sattler Wolfgang
Schweighofer Natascha
Stadelmeyer Elke
Sundl Monika
Windhager Reinhard
Windischhofer Werner
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Number of Figures: 6
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Abstract:
Although the liver is the primary site of cytokine-mediated expression of acute-phase serum amyloid A (SAA) protein, extrahepatic production has also been reported. Besides its role in amyloidosis and lipid homeostasis during the acute-phase, SAA has recently been assumed to contribute to bone and cartilage destruction. However, expression of SAA in human osteogenic tissue has not been studied. Therefore, we first show that SAA1 (coding for the major SAA isoform) but not SAA2 transcripts are expressed in human trabecular and cortical bone fractions and bone marrow. Next, we show expression of (i) IL-1, IL-6, and TNF receptor transcripts; (ii) the human homolog of SAA-activating factor-1 (SAF-1, a transcription factor involved in cytokine-mediated induction of SAA genes); and (iii) SAA1/2 transcripts in non-differentiated and, to a higher extent, in osteoblast-like differentiated human mesenchymal stem cells. Third, we provide evidence that human osteoblast-like cells of tumor origin (MG-63 and SAOS-2) express SAF-1 under basal conditions. SAA1/2 transcripts are expressed under basal conditions (SAOS-2) and cytokine-mediated conditions (MG-63 and SAOS-2). RT-PCR, Western blot analysis, and immunofluorescence technique confirmed cytokine-mediated expression of SAA on RNA and protein level in osteosarcoma cell lines while SAA4, a protein of unknown function, is constitutively expressed in all osteogenic tissues investigated.
Find related publications in this database (using NLM MeSH Indexing)
Acute-Phase Reaction - genetics
Aged -
Bone and Bones - cytology
Cell Differentiation -
Cells, Cultured -
Female -
Gene Expression - genetics
Humans -
Interleukin-1 - metabolism
Interleukin-6 - metabolism
Liver - metabolism
Male -
Molecular Sequence Data -
Osteoblasts - cytology
Osteosarcoma - genetics
RNA, Messenger - biosynthesis
Receptors, Tumor Necrosis Factor - metabolism
Serum Amyloid A Protein - biosynthesis
Stem Cells - cytology
Transcription, Genetic -
Tumor Markers, Biological -
Tumor Necrosis Factor-alpha - metabolism

Find related publications in this database (Keywords)
SAA
SAF-1
cancer
inflammation
FPRL-1/ALX
SR-BI
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