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SHR Neuro Krebs Kardio Lipid

Wiesenberger, G; Steinleitner, K; Malli, R; Graier, WF; Vormann, J; Schweyen, RJ; Stadler, JA.
Mg2+ deprivation elicits rapid Ca2+ uptake and activates Ca2+/calcineurin signaling in Saccharomyces cerevisiae.
Eukaryot Cell. 2007; 6(4): 592-599. [OPEN ACCESS]
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Autor/innen der Med Uni Graz:
Graier Wolfgang
Malli Roland

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Number of Figures: 6
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To learn about the cellular processes involved in Mg(2+) homeostasis and the mechanisms allowing cells to cope with low Mg(2+) availability, we performed RNA expression-profiling experiments and followed changes in gene activity upon Mg(2+) depletion on a genome-wide scale. A striking portion of genes up-regulated under Mg(2+) depletion are also induced by high Ca(2+) and/or alkalinization. Among the genes significantly up-regulated by Mg(2+) starvation, Ca(2+) stress, and alkalinization are ENA1 (encoding a P-type ATPase sodium pump) and PHO89 (encoding a sodium/phosphate cotransporter). We show that up-regulation of these genes is dependent on the calcineurin/Crz1p (calcineurin-responsive zinc finger protein) signaling pathway. Similarly to Ca(2+) stress, Mg(2+) starvation induces translocation of the transcription factor Crz1p from the cytoplasm into the nucleus. The up-regulation of ENA1 and PHO89 upon Mg(2+) starvation depends on extracellular Ca(2+). Using fluorescence resonance energy transfer microscopy, we demonstrate that removal of Mg(2+) results in an immediate increase in free cytoplasmic Ca(2+). This effect is dependent on external Ca(2+). The results presented indicate that Mg(2+) depletion in yeast cells leads to enhanced cellular Ca(2+) concentrations, which activate the Crz1p/calcineurin pathway. We provide evidence that calcineurin/Crz1p signaling is crucial for yeast cells to cope with Mg(2+) depletion stress.
Find related publications in this database (using NLM MeSH Indexing)
Calcineurin - metabolism
Calcium - metabolism
Calcium Signaling - drug effects
Cytoplasm - drug effects
Food Deprivation - drug effects
Gene Expression Regulation, Fungal - drug effects
Genome, Fungal - drug effects
Magnesium - metabolism
RNA, Messenger - genetics
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae Proteins - genetics
Transcription, Genetic - drug effects

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