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SHR Neuro Krebs Kardio Lipid

Kratzer, I; Wernig, K; Panzenboeck, U; Bernhart, E; Reicher, H; Wronski, R; Windisch, M; Hammer, A; Malle, E; Zimmer, A; Sattler, W.
Apolipoprotein A-I coating of protamine-oligonucleotide nanoparticles increases particle uptake and transcytosis in an in vitro model of the blood-brain barrier.
J Control Release. 2007; 117(3): 301-311. [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

 

Autor/innen der Med Uni Graz:
Bernhart Eva Maria
Hammer Astrid
Kratzer Ingrid
Malle Ernst
Panzenboeck Ute
Sattler Wolfgang
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Number of Figures: 8
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Abstract:
Drug delivery to the brain is severely restricted by formation of tight junctions between adjacent brain capillary endothelial cells (BCEC). In the present study we have evaluated the effects of protamine-oligonucleotide nanoparticles (proticles) on the functional properties of primary porcine BCEC and characterized uptake and transcytosis of proticles by these cells. Proticles had no adverse effects on BCEC properties relevant to blood-brain barrier (BBB) function. Transcytosis of (125)I-labeled proticles across polarized BCEC cultures occurred in a time- and concentration-dependent manner. As apolipoproteins were suggested to enhance cellular proticle uptake, proticle coating was performed with apoA-I, the major apolipoprotein component of high density lipoproteins. Adsorption of apoA-I on the surface of proticles resulted in significantly improved uptake and transcytosis properties as compared to uncoated proticles. ApoA-I coating enhanced proticle delivery to astrocytes in an in vitro model of the BBB almost twofold. Blocking of scavenger receptor class B, type I (the prime receptor for high density lipoprotein/apoA-I that is expressed on BCEC) reduced transcytosis of apoA-I-coated proticles to levels observed for uncoated proticles. Our data indicate that apoA-I-coating of proticles could be a feasible targeting technology to improve delivery across the BBB.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Apolipoprotein A-I - pharmacology
Astrocytes - metabolism
Blood-Brain Barrier - drug effects
Blotting, Western - drug effects
Brain - cytology
Brain Chemistry - drug effects
Chromatography, High Pressure Liquid - drug effects
Coculture Techniques - drug effects
Endothelial Cells - drug effects
Fluorescent Antibody Technique - drug effects
Humans - drug effects
Lipoproteins, HDL - chemistry
Nanoparticles - chemistry
Oligonucleotides - pharmacology
Particle Size - pharmacology
Protamines - pharmacology
Spectrometry, Mass, Electrospray Ionization - pharmacology
Swine - pharmacology
Tetrazolium Salts - pharmacology
Thiazoles - pharmacology

Find related publications in this database (Keywords)
HDL
SR-BI
proticles
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