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SHR Neuro Krebs Kardio Lipid

Sovic, A; Panzenboeck, U; Wintersperger, A; Kratzer, I; Hammer, A; Levak-Frank, S; Frank, S; Rader, DJ; Malle, E; Sattler, W.
Regulated expression of endothelial lipase by porcine brain capillary endothelial cells constituting the blood-brain barrier.
J NEUROCHEM. 2005; 94(1): 109-119.
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Autor/innen der Med Uni Graz:
Frank Saša
Hammer Astrid
Kratzer Ingrid
Levak Sanja
Malle Ernst
Panzenboeck Ute
Sattler Wolfgang
Wintersperger Andrea
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Abstract:
Normal neurological function depends on a constant supply of polyunsaturated fatty acids to the brain. A considerable proportion of essential fatty acids originates from lipoprotein-associated lipids that undergo uptake and/or catabolism at the blood-brain barrier (BBB). This study aimed at identifying expression and regulation of endothelial lipase (EL) in brain capillary endothelial cells (BCEC), major constituents of the BBB. Our results revealed that BCEC are capable of EL synthesis and secretion. Overexpression of EL resulted in enhanced hydrolysis of extracellular high-density lipoprotein (HDL)-associated sn-2-labeled [(14)C]20 : 4 phosphatidylcholine. [(14)C]20 : 4 was recovered in cellular lipids, indicating re-uptake and intracellular re-esterification. To investigate local regulation of EL in the cerebrovasculature, BCEC were cultured in the presence of peroxisome-proliferator activated receptor (PPAR)- and liver X receptor (LXR)-agonists, known to regulate HDL levels. These experiments revealed that 24(S)OH-cholesterol (a LXR agonist), bezafibrate (a PPARalpha agonist), or pioglitazone (a PPARgamma agonist) resulted in down-regulation of EL mRNA and protein levels. Our findings implicate that EL could generate fatty acids at the BBB for transport to deeper regions of the brain as building blocks for membrane phospholipids. In addition PPAR and LXR agonists appear to contribute to HDL homeostasis at the BBB by regulating EL expression.
Find related publications in this database (using NLM MeSH Indexing)
Animals -
Blood-Brain Barrier - enzymology
Brain - enzymology
Cells, Cultured - enzymology
Endothelium, Vascular - enzymology
Gene Expression Regulation, Enzymologic - physiology
Humans - physiology
Lipase - biosynthesis
Swine - biosynthesis

Find related publications in this database (Keywords)
bezafibrate
fatty acids
liver X receptor
pioglitazone
peroxisome-proliferator activated receptor
24(S)OH-cholesterol
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